ArticleBiophysical journal2009
Extracellular space volume measured by two-color pulsed dye infusion with microfiberoptic fluorescence photodetection.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Designed Cell-Penetrating Peptide Constructs for Inhibition of Pathogenic Protein Self-Assembly.Pharmaceutics · 2024Review
- Toward dynamic, anisotropic, high-resolution, and functional measurement in the brain extracellular space.Neurophotonics · 2022Review
- Characterizing extracellular diffusion properties using diffusion-weighted MRS of sucrose injected in mouse brain.NMR in biomedicine · 2021Article
- Reevaluation of Astrocyte-Neuron Energy Metabolism with Astrocyte Volume Fraction Correction: Impact on Cellular Glucose Oxidation Rates, Glutamate-Glutamine Cycle Energetics, Glycogen Levels and Utilization Rates vs. Exercising Muscle, and NaNeurochemical research · 2020Review
- Spatial Organization and Dynamics of the Extracellular Space in the Mouse Retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Current Techniques for Investigating the Brain Extracellular Space.Frontiers in neuroscience · 2020Review
- 20-HETE promotes glucose-stimulated insulin secretion in an autocrine manner through FFAR1.Nature communications · 2018Article
- Fluid balance concepts in medicine: Principles and practice.World journal of nephrology · 2018Review
- Controlled release for local delivery of drugs: barriers and models.Journal of controlled release : official journal of the Controlled Release Society · 2014Review
- Diffusion in the extracellular space in brain and tumors.Physical biology · 2013Article
- Extracellular sheets and tunnels modulate glutamate diffusion in hippocampal neuropil.The Journal of comparative neurology · 2013Article
- Shaping the synaptic signal: molecular mobility inside and outside the cleft.Trends in neurosciences · 2011Review
- In vivo two-photon uncaging of glutamate revealing the structure-function relationships of dendritic spines in the neocortex of adult mice.The Journal of physiology · 2011Article
- Microfiberoptic measurement of extracellular space volume in brain and tumor slices based on fluorescent dye partitioning.Biophysical journal · 2010Article
- 3D 23Na MRI of human skeletal muscle at 7 Tesla: initial experience.European radiology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The extracellular space (ECS) is the aqueous matrix surrounding cells in solid tissues. The only method to measure ECS volume fraction (alpha) in vivo has been tetramethylammonium iontophoresis, a technically challenging method developed more than 25 years ago. We report a simple, quantitative method to measure alpha by microfiberoptic fluorescence detection of a self-quenched green dye, calcein, and a reference red dye, sulforhodamine 101, after pulsed iontophoretic infusion. The idea is that the maximum increase in calcein fluorescence after iontophoresis is proportional to the aqueous volume into which the dye is deposited. We validated the method theoretically, and experimentally, using cell-embedded gels with specified alpha and ECS viscosity. Measurements in living mice gave alpha of 0.20 +/- 0.01 in brain, 0.13 +/- 0.02 in kidney and 0.074 +/- 0.01 in skeletal muscle. The technical simplicity of the "pulsed-infusion microfiberoptic photodetection" method developed here should allow elucidation of the relatively understudied biological roles of the ECS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.