ArticleProteomics2009
Subcellular proteomics of Trypanosoma cruzi reservosomes.
Article in Proteomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 78 citations in OpenAlex.
- A PQQ-dependent oxidoreductase inFrontiers in cellular and infection microbiology · 2026Article
- An Overview ofPathogens (Basel, Switzerland) · 2025Review
- Trypanosoma cruzi has Two Peptidyl-tRNA Hydrolases Showing Different Localization and Function.Acta parasitologica · 2025Article
- Contribution of microscopy to a better understanding of the anatomy of pathogenic protists.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Characterization of NovelInternational journal of molecular sciences · 2024Article
- Iron Uptake ControlsAntioxidants (Basel, Switzerland) · 2023Article
- Biogenesis of extracellular vesicles in protozoan parasites: The ESCRT complex in the trafficking fast lane?PLoS pathogens · 2023Review
- Transcriptomic analysis of the adaptation to prolonged starvation of the insect-dwellingFrontiers in cellular and infection microbiology · 2023Article
- Metabolic Alteration ofPathogens (Basel, Switzerland) · 2022Article
- Lipid droplets and the host-pathogen dynamic: FATal attraction?The Journal of cell biology · 2021Review
- Lopinavir and Nelfinavir Induce the Accumulation of Crystalloid Lipid Inclusions within the Reservosomes ofTropical medicine and infectious disease · 2021Article
- Biogenesis and Breakdown of Lipid Droplets in Pathological Conditions.Frontiers in cell and developmental biology · 2021Review
- Transcriptional remodeling during metacyclogenesis inVirulence · 2020Article
- Article
- Proteomic analysis reveals different composition of extracellular vesicles released by twoJournal of extracellular vesicles · 2018Article
- Lipid Droplet, a Key Player in Host-Parasite Interactions.Frontiers in immunology · 2018Review
- Genome sequencing reveals metabolic and cellular interdependence in an amoeba-kinetoplastid symbiosis.Scientific reports · 2017Article
- Article
- Trypanosoma cruzi Epimastigotes Are Able to Manage Internal Cholesterol Levels under Nutritional Lipid Stress Conditions.PloS one · 2015Article
- Unveiling the Trypanosoma cruzi Nuclear Proteome.PloS one · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Reservosomes are the endpoint of the endocytic pathway in Trypanosoma cruzi epimastigotes. These organelles have the particular ability to concentrate proteins and lipids obtained from medium together with the main proteolytic enzymes originated from the secretory pathway, being at the same time a storage organelle and the main site of protein degradation. Subcellular proteomics have been extensively used for profiling organelles in different cell types. Here, we combine cell fractionation and LC-MS/MS analysis to identify reservosome-resident proteins. Starting from a purified reservosome fraction, we established a protocol to isolate reservosome membranes. Transmission electron microscopy was applied to confirm the purity of the fractions. To achieve a better coverage of identified proteins we analyzed the fractions separately and combined the results. LC-MS/MS analysis identified in total 709 T. cruzi-specific proteins; of these, 456 had predicted function and 253 were classified as hypothetical proteins. We could confirm the presence of most of the proteins validated by previous work and identify new proteins from different classes such as enzymes, proton pumps, transport proteins, and others. The definition of the reservosome protein profile is a good tool to assess their molecular signature, identify molecular markers, and understand their relationship with different organelles.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.