ArticlePlant cell reports2009
Sequence analysis and functional characterization of the promoter of the Picea glauca Cinnamyl Alcohol Dehydrogenase gene in transgenic white spruce plants.
Article in Plant cell reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
- Genome-Wide Prediction of Transcription Start Sites in Conifers.International journal of molecular sciences · 2022Article
- Drought-induced ABA, HBMC plant biology · 2021Article
- The MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus.BMC plant biology · 2020Article
- Functional Analysis of theFrontiers in plant science · 2019Article
- Biosynthesis and Metabolic Fate of Phenylalanine in Conifers.Frontiers in plant science · 2016Review
- Large-scale screening of transcription factor-promoter interactions in spruce reveals a transcriptional network involved in vascular development.Journal of experimental botany · 2014Article
- Opposite action of R2R3-MYBs from different subgroups on key genes of the shikimate and monolignol pathways in spruce.Journal of experimental botany · 2014Article
- The cinnamyl alcohol dehydrogenase gene family in melon (Cucumis melo L.): bioinformatic analysis and expression patterns.PloS one · 2014Article
- The transcriptomics of secondary growth and wood formation in conifers.Molecular biology international · 2013Review
- Expression patterns of a cinnamyl alcohol dehydrogenase gene involved in lignin biosynthesis and environmental stress in Ginkgo biloba.Molecular biology reports · 2013Article
- Phylogeny and structure of the cinnamyl alcohol dehydrogenase gene family in Brachypodium distachyon.Journal of experimental botany · 2012Article
- Subgroup 4 R2R3-MYBs in conifer trees: gene family expansion and contribution to the isoprenoid- and flavonoid-oriented responses.Journal of experimental botany · 2010Article
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The enzyme Cinnamyl Alcohol Dehydrogenase (CAD) catalyses the last step of lignin monomer synthesis, and is considered as a molecular marker of cell wall lignification in different plants species. Here, we report the isolation and analysis of 5' flanking genomic DNA regions upstream to the CAD gene, from two conifers, i.e. white spruce (Picea glauca (Moench) Voss) and loblolly pine (Pinus taeda L.). Sequence comparisons with available CAD gene promoters from angiosperms highlighted the conservation of cis-elements matching MYB, WRKY and bHLH binding sites. Functional characterization of the P. glauca CAD promoter used P. glauca seedlings stably transformed with a DNA fragment of 1,163 base pairs (PgCAD) fused to the beta-glucuronidase (GUS) gene. Histochemical observations of different vegetative organs of the transgenic trees showed that this sequence was sufficient to drive GUS expression in lignifying tissues, and more specifically in differentiating xylem cells. Quantitative RT-PCR experiments also indicated that the native CAD gene was preferentially expressed in differentiating xylem both in stems and roots. In addition, GUS expression driven by the PgCAD promoter was wound-inducible which was consistent with the accumulation of CAD mRNA in response to jasmonate application and mechanical wounding. The spruce CAD promoter represents a valuable tool for research and biotechnology applications related to xylem and wood.
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