ArticleEukaryotic cell2009
Differential roles of the ChiB chitinase in autolysis and cell death of Aspergillus nidulans.
Article in Eukaryotic cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 71 citations in OpenAlex.
- Genome-Wide Identification and Analysis of Chitinase GH18 Gene Family inJournal of fungi (Basel, Switzerland) · 2025Article
- Expression profiles and characterization of microRNAs responding to chitin in Arthrobotrys oligospora.Archives of microbiology · 2024Article
- Different Putative Methyltransferases Have Different Effects on the Expression Patterns of Cellulolytic Genes.Journal of fungi (Basel, Switzerland) · 2023Article
- Velvet activated McrA plays a key role in cellular and metabolic development in Aspergillus nidulans.Scientific reports · 2020Article
- LysM Proteins Regulate Fungal Development and Contribute to Hyphal Protection and Biocontrol Traits inFrontiers in microbiology · 2020Article
- Transcriptomic and Functional Studies of the RGS Protein Rax1 in Aspergillus fumigatus.Pathogens (Basel, Switzerland) · 2019Article
- Fungal Biofilms: Inside Out.Microbiology spectrum · 2017Review
- Fungal chitinases: function, regulation, and potential roles in plant/pathogen interactions.Current genetics · 2016Review
- The N-acetylglucosamine catabolic gene cluster in Trichoderma reesei is controlled by the Ndt80-like transcription factor RON1.Molecular microbiology · 2016Article
- The carbon starvation response of the ectomycorrhizal fungus Paxillus involutus.FEMS microbiology ecology · 2015Article
- MrSkn7 controls sporulation, cell wall integrity, autolysis, and virulence in Metarhizium robertsii.Eukaryotic cell · 2015Article
- Article
- Elucidating how the saprophytic fungus Aspergillus nidulans uses the plant polyester suberin as carbon source.BMC genomics · 2014Article
- Absence of fks1p in lager brewing yeast results in aberrant cell wall composition and improved beer flavor stability.World journal of microbiology & biotechnology · 2014Article
- Article
- Article
- Distinct septin heteropolymers co-exist during multicellular development in the filamentous fungus Aspergillus nidulans.PloS one · 2014Article
- Extracellular DNA release acts as an antifungal resistance mechanism in mature Aspergillus fumigatus biofilms.Eukaryotic cell · 2013Article
- A p53-like transcription factor similar to Ndt80 controls the response to nutrient stress in the filamentous fungus, Aspergillus nidulans.F1000Research · 2013Article
- The putative guanine nucleotide exchange factor RicA mediates upstream signaling for growth and development in Aspergillus.Eukaryotic cell · 2012Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autolysis is a natural event that occurs in most filamentous fungi. Such self-degradation of fungal cells becomes a predominant phenomenon in the absence of the regulator of G protein signaling FlbA in Aspergillus nidulans. Among a number of potential hydrolytic enzymes in the A. nidulans genome, the secreted endochitinase ChiB was shown to play a major role in autolysis. In this report, we investigate the roles of ChiB in fungal autolysis and cell death processes through genetic, biochemical, and cellular analyses using a set of critical mutants. Determination of mycelial mass revealed that, while the flbA deletion (DeltaflbA) mutant autolyzed completely after a 3-day incubation, the DeltaflbA DeltachiB double mutant escaped from hyphal disintegration. These results indicate that ChiB is necessary for the DeltaflbA-induced autolysis. However, importantly, both DeltaflbA and DeltaflbA DeltachiB strains displayed dramatically reduced cell viability compared to the wild type. These imply that ChiB is dispensable for cell death and that autolysis and cell death are separate processes. Liquid chromatography-tandem mass spectrometry analyses of the proteins that accumulate at high levels in the DeltaflbA and DeltaflbA DeltachiB mutants identify chitinase (ChiB), dipeptidyl peptidase V (DppV), O-glycosyl compound hydrolase, beta-N-acetylhexosaminidase (NagA), and myo-inositol-1-phosphate synthase (InoB). Functional characterization of these four genes reveals that the deletion of nagA results in reduced cell death. A working model bridging G protein signaling and players in autolysis/cell death is proposed.
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