ArticleEukaryotic cell2009
Fungal functional genomics: tunable knockout-knock-in expression and tagging strategies.
Article in Eukaryotic cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Rhythmic nuclear import mediated by importins regulates the Neurospora circadian clock.Genetics · 2026Article
- Rhythmic Nuclear Import Mediated by Importins Regulates thebioRxiv : the preprint server for biology · 2026Article
- Fungal Innovations-Advancing Sustainable Materials, Genetics, and Applications for Industry.Journal of fungi (Basel, Switzerland) · 2025Review
- Transcriptional rewiring of an evolutionarily conserved circadian clock.The EMBO journal · 2024Article
- Identification and Characterization of an Antifungal GeneInternational journal of molecular sciences · 2023Article
- Article
- Circadian oscillations ineLife · 2022Article
- An Inactivation Switch Enables Rhythms in aInternational journal of molecular sciences · 2019Article
- Light-regulated promoters for tunable, temporal, and affordable control of fungal gene expression.Applied microbiology and biotechnology · 2018Review
- Article
- Modulation of Circadian Gene Expression and Metabolic Compensation by the RCO-1 Corepressor of Neurospora crassa.Genetics · 2016Article
- The frequency natural antisense transcript first promotes, then represses, frequency gene expression via facultative heterochromatin.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Bright to dim oscillatory response of the Neurospora circadian oscillator.Journal of biological rhythms · 2014Article
- Circadian rhythms synchronize mitosis in Neurospora crassa.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Establishment of an efficient RNA silencing system in Trichoderma koningii using DsRed as a reporter.Folia microbiologica · 2013Article
- Microtubule-based transport in filamentous fungi.Current opinion in microbiology · 2012Review
- High-resolution spatiotemporal analysis of gene expression in real time: in vivo analysis of circadian rhythms in Neurospora crassa using a FREQUENCY-luciferase translational reporter.Fungal genetics and biology : FG & B · 2012Article
- The circadian clock of Neurospora crassa.FEMS microbiology reviews · 2012Review
- Fungal gene expression on demand: an inducible, tunable, and metabolism-independent expression system for Aspergillus niger.Applied and environmental microbiology · 2011Article
- Genetic and molecular characterization of a cryptochrome from the filamentous fungus Neurospora crassa.Eukaryotic cell · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Strategies for promoting high-efficiency homologous gene replacement have been developed and adopted for many filamentous fungal species. The next generation of analysis requires the ability to manipulate gene expression and to tag genes expressed from their endogenous loci. Here we present a suite of molecular tools that provide versatile solutions for fungal high-throughput functional genomics studies based on locus-specific modification of any target gene. Additionally, case studies illustrate caveats to presumed overexpression constructs. A tunable expression system and different tagging strategies can provide valuable phenotypic information for uncharacterized genes and facilitate the analysis of essential loci, an emerging problem in systematic deletion studies of haploid organisms.
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