ArticleJournal of experimental botany2009
Characterization of leaf apoplastic peroxidases and metabolites in Vigna unguiculata in response to toxic manganese supply and silicon.
Article in Journal of experimental botany, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 80 citations in OpenAlex.
- Abiotic stress responses in forage crops and grasses: the role of secondary metabolites and biotechnological interventions.Frontiers in plant science · 2025Review
- Identification of mungbean yellow mosaic India virus and susceptibility-related metabolites in the apoplast of mung bean leaves.Plant cell reports · 2024Article
- OMICS in Fodder Crops: Applications, Challenges, and Prospects.Current issues in molecular biology · 2022Review
- Manganese toxicity disrupts indole acetic acid homeostasis and suppresses the COScientific reports · 2021Article
- Advances in the Mechanisms of Plant Tolerance to Manganese Toxicity.International journal of molecular sciences · 2019Review
- A Simple Method for Measuring Apoplast Hydration and Collecting Apoplast Contents.Plant physiology · 2019Article
- Article
- Altered Expression of a Malate-Permeable Anion Channel, OsALMT4, Disrupts Mineral Nutrition.Plant physiology · 2017Article
- Protein Dynamics in the Plant Extracellular Space.Proteomes · 2016Review
- Silicon decreases both uptake and root-to-shoot translocation of manganese in rice.Journal of experimental botany · 2016Article
- Silicon improves salt tolerance by increasing root water uptake in Cucumis sativus L.Plant cell reports · 2015Article
- Silicon in vascular plants: uptake, transport and its influence on mineral stress under acidic conditions.Planta · 2015Review
- Two-dimensional phos-tag zymograms for tracing phosphoproteins by activity in-gel staining.Frontiers in plant science · 2015Article
- Article
- Statistical methods for the analysis of high-throughput metabolomics data.Computational and structural biotechnology journal · 2013Review
- Review
- Functional associations between the metabolome and manganese tolerance in Vigna unguiculata.Journal of experimental botany · 2012Article
- Enolases: storage compounds in seeds? Evidence from a proteomic comparison of zygotic and somatic embryos of Cyclamen persicum Mill.Plant molecular biology · 2011Article
- Soybean metabolites regulated in root hairs in response to the symbiotic bacterium Bradyrhizobium japonicum.Plant physiology · 2010Article
- Physiological and proteomic characterization of manganese sensitivity and tolerance in rice (Oryza sativa) in comparison with barley (Hordeum vulgare).Annals of botany · 2010Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous work suggested that the apoplastic phenol composition and its interaction with apoplastic class III peroxidases (PODs) are decisive in the development or avoidance of manganese (Mn) toxicity in cowpea (Vigna unguiculata L.). This study characterizes apoplastic PODs with particular emphasis on the activities of specific isoenzymes and their modulation by phenols in the Mn-sensitive cowpea cultivar TVu 91 as affected by Mn and silicon (Si) supply. Si reduced Mn-induced toxicity symptoms without affecting the Mn uptake. Blue Native-PAGE combined with Nano-LC-MS/MS allowed identification of a range of POD isoenzymes in the apoplastic washing fluid (AWF). In Si-treated plants Mn-mediated induction of POD activity was delayed. Four POD isoenzymes eluted from the BN gels catalysed both H(2)O(2)-consuming and H(2)O(2)-producing activity with pH optima at 6.5 and 5.5, respectively. Four phenols enhanced NADH-peroxidase activity of these isoenzymes in the presence of Mn(2+) (p-coumaric=vanillic>>benzoic>ferulic acid). p-Coumaric acid-enhanced NADH-peroxidase activity was inhibited by ferulic acid (50%) and five other phenols (50-90%). An independent component analysis (ICA) of the total and apoplastic GC-MS-based metabolome profile showed that Mn, Si supply, and the AWF fraction (AWF(H(2)O), AWF(NaCl)) significantly changed the metabolite composition. Extracting non-polar metabolites from the AWF allowed the identification of phenols. Predominantly NADH-peroxidase activity-inhibiting ferulic acid appeared to be down-regulated in Mn-sensitive (+Mn, -Si) and up-regulated in Mn-tolerant (+Si) leaf tissue. The results presented here support the previously hypothesized role of apoplastic NADH-peroxidase and its activity-modulating phenols in Mn toxicity and Si-enhanced Mn tolerance.
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