ArticleJournal of bacteriology2009
Identification and characterization of a novel multidrug resistance operon, mdtRP (yusOP), of Bacillus subtilis.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
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- Bacterial diversity and the antimicrobial resistome in the southwestern highlands of Saudi Arabia.Saudi journal of biological sciences · 2022Article
- Impact of activation of neotrehalosadiamine/kanosamine biosynthetic pathway on the metabolism ofJournal of bacteriology · 2021Article
- Impact of commonly used drugs on the composition and metabolic function of the gut microbiota.Nature communications · 2020Article
- Amphotericin B Specifically Induces the Two-Component System LnrJK: Development of a Novel Whole-Cell Biosensor for the Detection of Amphotericin-Like Polyenes.Frontiers in microbiology · 2020Article
- The Bacillus subtilis tyrZ gene encodes a highly selective tyrosyl-tRNA synthetase and is regulated by a MarR regulator and T box riboswitch.Journal of bacteriology · 2015Article
- Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis.Applied and environmental microbiology · 2011Article
- Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2010 update).EFSA journal. European Food Safety Authority · 2010Article
- LVIS553 transcriptional regulator specifically recognizes novobiocin as an effector molecule.The Journal of biological chemistry · 2010Article
- ScoC regulates bacilysin production at the transcription level in Bacillus subtilis.Journal of bacteriology · 2009Article
- Review
- Modulation of Bacterial Multidrug Resistance Efflux Pumps of the Major Facilitator Superfamily.International journal of bacteriologyArticle
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using comparative genome sequencing analysis, we identified a novel mutation in Bacillus subtilis that confers a low level of resistance to fusidic acid. This mutation was located in the mdtR (formerly yusO) gene, which encodes a MarR-type transcriptional regulator, and conferred a low level of resistance to several antibiotics, including novobiocin, streptomycin, and actinomycin D. Transformation experiments showed that this mdtR mutation was responsible for multidrug resistance. Northern blot analysis revealed that the downstream gene mdtP (formerly yusP), which encodes a multidrug efflux transporter, is cotranscribed with mdtR as an operon. Disruption of the mdtP gene completely abolished the multidrug resistance phenotype observed in the mdtR mutant. DNase I footprinting and primer extension analyses demonstrated that the MdtR protein binds directly to the mdtRP promoter, thus leading to repression of its transcription. Moreover, gel mobility shift analysis indicated that an Arg83 --> Lys or Ala67 --> Thr substitution in MdtR significantly reduces binding affinity to DNA, resulting in derepression of mdtRP transcription. Low concentrations of fusidic acid induced the expression of mdtP, although the level of mdtP expression was much lower than that in the mdtR disruptant. These findings indicate that the MdtR protein is a repressor of the mdtRP operon and that the MdtP protein functions as a multidrug efflux transporter in B. subtilis.
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