ArticleJournal of bacteriology2009
Whole-genome tiling array analysis of Mycobacterium leprae RNA reveals high expression of pseudogenes and noncoding regions.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 59 citations in OpenAlex.
- Loss to gain: pseudogenes in microorganisms, focusing on eubacteria, and their biological significance.Applied microbiology and biotechnology · 2024Review
- MicroRNA biomarkers in leprosy: insights from the Northern Brazilian Amazon population and their implications in disease immune-physiopathology.Frontiers in genetics · 2024Article
- Pathogenicity and virulence ofVirulence · 2022Review
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- Multi-omics Analysis Sheds Light on the Evolution and the Intracellular Lifestyle Strategies of Spotted Fever GroupFrontiers in microbiology · 2017Article
- Differential Expression of MicroRNAs in Leprosy Skin Lesions.Frontiers in immunology · 2017Article
- A Transcriptome Map of Actinobacillus pleuropneumoniae at Single-Nucleotide Resolution Using Deep RNA-Seq.PloS one · 2016Article
- Genome-wide analyses in bacteria show small-RNA enrichment for long and conserved intergenic regions.Journal of bacteriology · 2015Article
- Genome-Wide Screening of mRNA Expression in Leprosy Patients.Frontiers in genetics · 2015Article
- PCR-based techniques for leprosy diagnosis: from the laboratory to the clinic.PLoS neglected tropical diseases · 2014Review
- Thyroglobulin increases thyroid cell proliferation via the suppression of specific microRNAs.Molecular endocrinology (Baltimore, Md.) · 2014Article
- Small RNAs in mycobacteria: an unfolding story.Frontiers in cellular and infection microbiology · 2014Review
- Comprehensive analysis of prokaryotes in environmental water using DNA microarray analysis and whole genome amplification.Pathogens (Basel, Switzerland) · 2013Article
- Identification of microRNAs that mediate thyroid cell growth induced by TSH.Molecular endocrinology (Baltimore, Md.) · 2012Article
- Global discovery of small noncoding RNAs in pathogenic Yersinia species.Advances in experimental medicine and biology · 2012Review
- RNA-seq based transcriptional map of bovine respiratory disease pathogen "Histophilus somni 2336".PloS one · 2012Article
- A genome-wide identification analysis of small regulatory RNAs in Mycobacterium tuberculosis by RNA-Seq and conservation analysis.PloS one · 2012Article
- Clofazimine modulates the expression of lipid metabolism proteins in Mycobacterium leprae-infected macrophages.PLoS neglected tropical diseases · 2012Article
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Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whole-genome sequence analysis of Mycobacterium leprae has revealed a limited number of protein-coding genes, with half of the genome composed of pseudogenes and noncoding regions. We previously showed that some M. leprae pseudogenes are transcribed at high levels and that their expression levels change following infection. In order to clarify the RNA expression profile of the M. leprae genome, a tiling array in which overlapping 60-mer probes cover the entire 3.3-Mbp genome was designed. The array was hybridized with M. leprae RNA from the SHR/NCrj-rnu nude rat, and the results were compared to results from an open reading frame array and confirmed by reverse transcription-PCR. RNA expression was detected from genes, pseudogenes, and noncoding regions. The signal intensities obtained from noncoding regions were higher than those from pseudogenes. Expressed noncoding regions include the M. leprae unique repetitive sequence RLEP and other sequences without any homology to known functional noncoding RNAs. Although the biological functions of RNA transcribed from M. leprae pseudogenes and noncoding regions are not known, RNA expression analysis will provide insights into the bacteriological significance of the species. In addition, our study suggests that M. leprae will be a useful model organism for the study of the molecular mechanism underlying the creation of pseudogenes and the role of microRNAs derived from noncoding regions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.