ArticleApplied and environmental microbiology2009
prtH2, not prtH, is the ubiquitous cell wall proteinase gene in Lactobacillus helveticus.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 63 citations in OpenAlex.
- Research progress on peptides that inhibit melanin synthesis.Frontiers in pharmacology · 2025Review
- Molecular and biotechnological characteristics of proteolytic activity fromAIMS microbiology · 2023Article
- The Enzyme Gene Expression of Protein Utilization and Metabolism byMicroorganisms · 2022Article
- Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins fromFrontiers in microbiology · 2022Article
- Little Impact of NaCl Reduction in Swiss-Type Cheese.Frontiers in nutrition · 2022Article
- Article
- The Impact of the Adjunct Heat-Treated Starter Culture andAnimals : an open access journal from MDPI · 2021Article
- Characteristics of the Proteolytic Enzymes Produced by Lactic Acid Bacteria.Molecules (Basel, Switzerland) · 2021Review
- Metabolism Characteristics of Lactic Acid Bacteria and the Expanding Applications in Food Industry.Frontiers in bioengineering and biotechnology · 2021Review
- Use ofInternational journal of food science · 2021Article
- Use of Mass Spectrometry to Profile Peptides in Whey Protein Isolate Medium Fermented byFrontiers in nutrition · 2019Article
- Distribution of Cell Envelope Proteinases Genes among Polish Strains of Lactobacillus helveticus.Polish journal of microbiology · 2018Article
- Production of Bioactive Peptides byFrontiers in microbiology · 2018Review
- The S-layer Associated Serine Protease Homolog PrtX Impacts Cell Surface-Mediated Microbe-Host Interactions ofFrontiers in microbiology · 2017Article
- Effect of culturing conditions on the expression of key enzymes in the proteolytic system of Lactobacillus bulgaricus.Journal of Zhejiang University. Science. B · 2015Article
- Identification of Antihypertensive Peptides Derived from Low Molecular Weight Casein Hydrolysates Generated during Fermentation by Bifidobacterium longum KACC 91563.Korean journal for food science of animal resources · 2015Article
- The naturally competent strain Streptococcus thermophilus LMD-9 as a new tool to anchor heterologous proteins on the cell surface.Microbial cell factories · 2014Article
- Lactobacillus helveticus: the proteolytic system.Frontiers in microbiology · 2013Article
- Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus.Frontiers in microbiology · 2013Article
- The fast milk acidifying phenotype of Streptococcus thermophilus can be acquired by natural transformation of the genomic island encoding the cell-envelope proteinase PrtS.Microbial cell factories · 2011Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactobacillus helveticus strains possess an efficient proteolytic system that releases peptides which are essential for lactobacillus growth in various fermented dairy products and also affect textural properties or biological activities. Cell envelope proteinases (CEPs) are bacterial enzymes that hydrolyze milk proteins. In the case of L. helveticus, two CEPs with low percentages of amino acid identity have been described, i.e., PrtH and PrtH2. However, the distribution of the genes that encode CEPs still remains unclear, rendering it difficult to further control the formation of particular peptides. This study evaluated the diversity of genes that encode CEPs in a collection of strains of L. helveticus isolated from various biotopes, both in terms of the presence or absence of these genes and in terms of nucleotide sequence, and studied their transcription in dairy matrices. After defining three sets of primers for both the prtH and prtH2 genes, we studied the distribution of the genes by using PCR and Southern blotting experiments. The prtH2 gene was ubiquitous in the 29 strains of L. helveticus studied, whereas only 18 of them also exhibited the prtH gene. Sequencing of a 350-bp internal fragment of these genes revealed the existence of intraspecific diversity. Finally, expression of these two CEP-encoding genes was followed during the growth in dairy matrices of two strains, ITG LH77 and CNRZ32, which possess one and two CEP-encoding genes, respectively. Both genes were shown to be expressed by L. helveticus at each stage of growth in milk and at different stages of mini-Swiss-type cheese making and ripening.
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