ArticleThe Journal of biological chemistry2009
Identification of PpoA from Aspergillus nidulans as a fusion protein of a fatty acid heme dioxygenase/peroxidase and a cytochrome P450.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 97 citations in OpenAlex.
- Catalytic and structural comparisons of linoleate dioxygenases and their cytochrome P450 companions with enzymes of the cyclooxygenase cascade.The Journal of biological chemistry · 2026Review
- Fatty acids in filamentous pathogenic fungi: Multidimensional regulation and pathogenic mechanisms.Virulence · 2025Review
- Genome-Wide Analysis of the Cytochrome P450 Monooxygenases in the Lichenized Fungi of the ClassMicroorganisms · 2023Article
- Pezizomycetes Genomes Reveal Diverse P450 Complements Characteristic of Saprotrophic and Ectomycorrhizal Lifestyles.Journal of fungi (Basel, Switzerland) · 2023Article
- Unfolded Protein Response and Scaffold Independent Pheromone MAP Kinase Signaling ControlJournal of fungi (Basel, Switzerland) · 2021Article
- Combined analysis of microbial community and microbial metabolites based on untargeted metabolomics during pig manure composting.Biodegradation · 2021Article
- Comparative analyses and structural insights of the novel cytochrome P450 fusion protein family CYP5619 in Oomycetes.Scientific reports · 2018Article
- Genome sequence of the filamentous soil fungus Chaetomium cochliodes reveals abundance of genes for heme enzymes from all peroxidase and catalase superfamilies.BMC genomics · 2016Article
- Production of cross-kingdom oxylipins by pathogenic fungi: An update on their role in development and pathogenicity.Journal of microbiology (Seoul, Korea) · 2016Review
- Characterization of a novel 8R,11S-linoleate diol synthase from Penicillium chrysogenum by identification of its enzymatic products.Journal of lipid research · 2016Article
- Article
- Diversity and evolution of cytochrome P450 monooxygenases in Oomycetes.Scientific reports · 2015Article
- Dynamics of the lipid droplet proteome of the Oleaginous yeast rhodosporidium toruloides.Eukaryotic cell · 2015Article
- Genome-Wide Annotation and Comparative Analysis of Cytochrome P450 Monooxygenases in Basidiomycete Biotrophic Plant Pathogens.PloS one · 2015Article
- LDS1-produced oxylipins are negative regulators of growth, conidiation and fumonisin synthesis in the fungal maize pathogen Fusarium verticillioides.Frontiers in microbiology · 2014Article
- Discovery of a linoleate 9S-dioxygenase and an allene oxide synthase in a fusion protein of Fusarium oxysporum.Journal of lipid research · 2013Article
- Unusual cytochrome p450 enzymes and reactions.The Journal of biological chemistry · 2013Review
- Expression of fusion proteins of Aspergillus terreus reveals a novel allene oxide synthase.The Journal of biological chemistry · 2013Article
- Microbial cytochromes P450: biodiversity and biotechnology. Where do cytochromes P450 come from, what do they do and what can they do for us?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Origins of P450 diversity.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The homothallic ascomycete Aspergillus nidulans serves as model organism for filamentous fungi because of its ability to propagate with both asexual and sexual life cycles, and fatty acid-derived substances regulate the balance between both cycles. These so-called psi (precocious sexual inducer) factors are produced by psi factor-producing oxygenases (Ppo enzymes). Bioinformatic analysis predicted the presence of two different heme domains in Ppo proteins: in the N-terminal region, a fatty acid heme dioxygenase/peroxidase domain is predicted, whereas in the C-terminal region, a P450 heme thiolate domain is predicted. To analyze the reaction catalyzed by Ppo enzymes, PpoA was expressed in Escherichia coli as an active enzyme. The protein was purified by 62-fold and identified as a homotetrameric ferric heme protein that metabolizes mono- as well as polyunsaturated C(16) and C(18) fatty acids at pH approximately 7.25. The presence of thiolate-ligated heme was confirmed on the basis of sequence alignments and the appearance of a characteristic 450 nm CO-binding spectrum. Studies on its reaction mechanism revealed that PpoA uses different heme domains to catalyze two separate reactions. Within the heme peroxidase domain, linoleic acid is oxidized to (8R)-hydroperoxyoctadecadienoic acid by abstracting a H-atom from C-8 of the fatty acid, yielding a carbon-centered radical that reacts with molecular dioxygen. In the second reaction step, 8-hydroperoxyoctadecadienoic acid is isomerized within the P450 heme thiolate domain to 5,8-dihydroxyoctadecadienoic acid. We identify PpoA as a bifunctional P450 fusion protein that uses a previously unknown reaction mechanism for forming psi factors.
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