ArticleVirology2009
Characterization of a myristoylated, monomeric HIV Gag protein.
Article in Virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 23 citations in OpenAlex.
- Impact of specific productivity and operation mode upon the biophysical properties of HIV-1 Gag-based virus-like particles.Applied microbiology and biotechnology · 2025Article
- Review
- Development and Preclinical Evaluation of an Integrase Defective Lentiviral Vector Vaccine Expressing the HIVACAT T Cell Immunogen in Mice.Molecular therapy. Methods & clinical development · 2020Article
- HIV-1 Gag as an Antiviral Target: Development of Assembly and Maturation Inhibitors.Current topics in medicinal chemistry · 2016Review
- Post-translational intracellular trafficking determines the type of immune response elicited by DNA vaccines expressing Gag antigen of Human Immunodeficiency Virus Type 1 (HIV-1).Human vaccines & immunotherapeutics · 2013Article
- Clustering and mobility of HIV-1 Env at viral assembly sites predict its propensity to induce cell-cell fusion.Journal of virology · 2013Article
- HIV-1 Gag associates with specific uropod-directed microdomains in a manner dependent on its MA highly basic region.Journal of virology · 2013Article
- Molecular mechanism of arenavirus assembly and budding.Viruses · 2012Review
- Role of the HIV-1 Matrix Protein in Gag Intracellular Trafficking and Targeting to the Plasma Membrane for Virus Assembly.Frontiers in microbiology · 2012Article
- Dynamic Association between HIV-1 Gag and Membrane Domains.Molecular biology international · 2012Article
- The cellular protein lyric interacts with HIV-1 Gag.Journal of virology · 2011Article
- Rous sarcoma virus gag has no specific requirement for phosphatidylinositol-(4,5)-bisphosphate for plasma membrane association in vivo or for liposome interaction in vitro.Journal of virology · 2011Article
- Gag induces the coalescence of clustered lipid rafts and tetraspanin-enriched microdomains at HIV-1 assembly sites on the plasma membrane.Journal of virology · 2011Article
- New insights into HIV assembly and trafficking.Physiology (Bethesda, Md.) · 2011Review
- FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ.Chemphyschem : a European journal of chemical physics and physical chemistry · 2011Article
- Binding of calmodulin to the HIV-1 matrix protein triggers myristate exposure.The Journal of biological chemistry · 2010Article
- Myristate exposure in the human immunodeficiency virus type 1 matrix protein is modulated by pH.Biochemistry · 2010Article
- HIV-1 assembly differentially alters dynamics and partitioning of tetraspanins and raft components.Traffic (Copenhagen, Denmark) · 2010Article
- Nucleocapsid promotes localization of HIV-1 gag to uropods that participate in virological synapses between T cells.PLoS pathogens · 2010Article
- Relationships between plasma membrane microdomains and HIV-1 assembly.Biology of the cell · 2010Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The process of HIV assembly requires extensive homomultimerization of the Gag polyprotein on cellular membranes to generate the nascent particle bud. Here we generated a full-length, monomeric Gag polyprotein bearing mutations that eliminated multimerization in living cells as indicated by fluorescence resonance energy transfer (FRET). Monomeric Gag resembled non-myristoylated Gag in its weak membrane binding characteristics and lack of association with detergent-resistant membranes (DRMs or lipid rafts). Monomeric Gag failed to assemble virus-like particles, but was inefficiently rescued into particles by wildtype Gag through the influence of the matrix domain. The subcellular distribution of monomeric Gag was remarkably different than either non-myristoylated Gag or wildtype Gag. Monomeric Gag was found on intracellular membranes and at the plasma membrane, where it highlighted plasma membrane extensions and ruffles. This study indicates that monomeric Gag can traffic to assembly sites in the cell, where it interacts weakly with membranes.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.