ArticleEpigenetics & chromatin2009
Bisulfite-based epityping on pooled genomic DNA provides an accurate estimate of average group DNA methylation.
Article in Epigenetics & chromatin, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- A cost-effective method for combining the power of genetic and epigenetic selection in animal production.Environmental epigenetics · 2025Article
- Exertional heat stroke causes long-term skeletal muscle epigenetic reprogramming, altered gene expression, and impaired satellite cell function in mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2024Article
- Whole-Genome DNA Methylation Dynamics of Sheep Preimplantation Embryo Investigated by Single-Cell DNA Methylome Sequencing.Frontiers in genetics · 2021Article
- Disease Activity-Associated Alteration of mRNA mFrontiers in cell and developmental biology · 2020Article
- Article
- DNA methylation assessment from human slow- and fast-twitch skeletal muscle fibers.Journal of applied physiology (Bethesda, Md. : 1985) · 2017Article
- Genome-wide alteration in DNA hydroxymethylation in the sperm from bisphenol A-exposed men.PloS one · 2017Article
- Whole-miRNome profiling identifies prognostic serum miRNAs in esophageal adenocarcinoma: the influence of Helicobacter pylori infection status.Carcinogenesis · 2015Article
- DNA methylation levels are highly correlated between pooled samples and averaged values when analysed using the Infinium HumanMethylation450 BeadChip array.Clinical epigenetics · 2015Article
- Whole-genome bisulfite sequencing of cell-free DNA identifies signature associated with metastatic breast cancer.Clinical epigenetics · 2015Article
- Genome-wide methylation analysis identifies differentially methylated CpG loci associated with severe obesity in childhood.Epigenetics · 2015Article
- Epigenome-wide association study reveals longitudinally stable DNA methylation differences in CD4+ T cells from children with IgE-mediated food allergy.Epigenetics · 2014Article
- Epigenetic patterns of two gene promoters (TNF-α and PON) in stroke considering obesity condition and dietary intake.Journal of physiology and biochemistry · 2014Article
- Altered DNA methylation status of human brain derived neurotrophis factor gene could be useful as biomarker of depression.American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics · 2014Article
- Single-base resolution of mouse offspring brain methylome reveals epigenome modifications caused by gestational folic acid.Epigenetics & chromatin · 2014Article
- Gender-specific DNA methylome analysis of a Han Chinese longevity population.BioMed research international · 2014Article
- Genome-wide analysis of DNA methylation in human amnion.TheScientificWorldJournal · 2013Article
- Article
- Postnatal development- and age-related changes in DNA-methylation patterns in the human genome.Nucleic acids research · 2012Article
- Genome-scale profiling reveals a subset of genes regulated by DNA methylation that program somatic T-cell phenotypes in humans.Genes and immunity · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundDNA methylation plays a vital role in normal cellular function, with aberrant methylation signatures being implicated in a growing number of human pathologies and complex human traits. Methods based on the modification of genomic DNA with sodium bisulfite are considered the 'gold-standard' for DNA methylation profiling on genomic DNA; however, they require relatively large amounts of DNA and may be prohibitively expensive when used on the large sample sizes necessary to detect small effects. We propose that a high-throughput DNA pooling approach will facilitate the use of emerging methylomic profiling techniques in large samples.
resultsCompared with data generated from 89 individual samples, our analysis of 205 CpG sites spanning nine independent regions of the genome demonstrates that DNA pools can be used to provide an accurate and reliable quantitative estimate of average group DNA methylation. Comparison of data generated from the pooled DNA samples with results averaged across the individual samples comprising each pool revealed highly significant correlations for individual CpG sites across all nine regions, with an average overall correlation across all regions and pools of 0.95 (95% bootstrapped confidence intervals: 0.94 to 0.96).
conclusionIn this study we demonstrate the validity of using pooled DNA samples to accurately assess group DNA methylation averages. Such an approach can be readily applied to the assessment of disease phenotypes reducing the time, cost and amount of DNA starting material required for large-scale epigenetic analyses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.