ArticlePloS one2009
The Werner syndrome helicase/exonuclease processes mobile D-loops through branch migration and degradation.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 48 citations in OpenAlex.
- Synthetic Lethal Interactions of RECQ Helicases.Trends in cancer · 2021Review
- Human RecQ Helicases in DNA Double-Strand Break Repair.Frontiers in cell and developmental biology · 2021Review
- Review
- Article
- Article
- RecQ and Fe-S helicases have unique roles in DNA metabolism dictated by their unwinding directionality, substrate specificity, and protein interactions.Biochemical Society transactions · 2018Review
- Werner syndrome: Clinical features, pathogenesis and potential therapeutic interventions.Ageing research reviews · 2017Review
- Telomere-associated aging disorders.Ageing research reviews · 2017Review
- Single-molecule imaging reveals a common mechanism shared by G-quadruplex-resolving helicases.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The biology of DHX9 and its potential as a therapeutic target.Oncotarget · 2016Review
- An Overview of the Molecular Mechanisms of Recombinational DNA Repair.Cold Spring Harbor perspectives in biology · 2015Review
- Preventing replication fork collapse to maintain genome integrity.DNA repair · 2015Review
- Telomeric overhang length determines structural dynamics and accessibility to telomerase and ALT-associated proteins.Structure (London, England : 1993) · 2014Article
- Human RECQL1 participates in telomere maintenance.Nucleic acids research · 2014Article
- Human RecQ helicases in DNA repair, recombination, and replication.Annual review of biochemistry · 2014Review
- Replication stress induces specific enrichment of RECQ1 at common fragile sites FRA3B and FRA16D.Molecular cancer · 2013Article
- Roles of DNA helicases in the mediation and regulation of homologous recombination.Advances in experimental medicine and biology · 2013Article
- Werner syndrome protein suppresses the formation of large deletions during the replication of human telomeric sequences.Cell cycle (Georgetown, Tex.) · 2012Article
- SMARCAL1 catalyzes fork regression and Holliday junction migration to maintain genome stability during DNA replication.Genes & development · 2012Article
- DNA end resection controls the balance between homologous and illegitimate recombination in Escherichia coli.PloS one · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
RecQ DNA helicases are critical for preserving genome integrity. Of the five RecQ family members identified in humans, only the Werner syndrome protein (WRN) possesses exonuclease activity. Loss of WRN causes the progeroid disorder Werner syndrome which is marked by cancer predisposition. Cellular evidence indicates that WRN disrupts potentially deleterious intermediates in homologous recombination (HR) that arise in genomic and telomeric regions during DNA replication and repair. Precisely how the WRN biochemical activities process these structures is unknown, especially since the DNA unwinding activity is poorly processive. We generated biologically relevant mobile D-loops which mimic the initial DNA strand invasion step in HR to investigate whether WRN biochemical activities can disrupt this joint molecule. We show that WRN helicase alone can promote branch migration through an 84 base pair duplex region to completely displace the invading strand from the D-loop. However, substrate processing is altered in the presence of the WRN exonuclease activity which degrades the invading strand both prior to and after release from the D-loop. Furthermore, telomeric D-loops are more refractory to disruption by WRN, which has implications for tighter regulation of D-loop processing at telomeres. Finally, we show that WRN can recognize and initiate branch migration from both the 5' and 3' ends of the invading strand in the D-loops. These findings led us to propose a novel model for WRN D-loop disruption. Our biochemical results offer an explanation for the cellular studies that indicate both WRN activities function in processing HR intermediates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.