ArticlePLoS pathogens2009
Natural transformation of helicobacter pylori involves the integration of short DNA fragments interrupted by gaps of variable size.
Article in PLoS pathogens, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 79 citations in OpenAlex.
- Review
- Low-Abundance and FragmentaryBiomolecules · 2026Article
- Within-host diversity and phased variant analysis reveal structures and recombination of Helicobacter pylori subpopulations in stomach.GigaScience · 2026Article
- High-throughput conjugation reveals strain specific recombination patterns enabling precise trait mapping in Escherichia coli.PLoS genetics · 2025Article
- Impact of Natural Transformation on the Acquisition of Novel Genes in Bacteria.Molecular biology and evolution · 2025Article
- A more rapid method for transformation ofmSphere · 2025Article
- Intragenomic conflicts with plasmids and chromosomal mobile genetic elements drive the evolution of natural transformation within species.PLoS biology · 2024Article
- Reduction of UreB and CagA expression level by siRNA construct in Helicobacter pylori strain SS1.BMC microbiology · 2023Article
- Genome-wide transformation reveals extensive exchange across closely related Bacillus species.Nucleic acids research · 2023Article
- Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach.Nature communications · 2023Article
- Catch me if you can: capturing microbial community transformation by extracellular DNA using Hi-C sequencing.Antonie van Leeuwenhoek · 2023Article
- Article
- Recombination resolves the cost of horizontal gene transfer in experimental populations ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Primary antibiotic resistance of Helicobacter pylori isolates is twofold more frequent in HIV-positive than HIV-negative individuals: A descriptive observational study.MicrobiologyOpen · 2021Observational
- Experimental Evolution ofGenetics · 2020Article
- Article
- Recombination-independent rapid convergent evolution of the gastric pathogen Helicobacter pylori.BMC genomics · 2018Article
- A genomic view of experimental intraspecies and interspecies transformation of a rifampicin-resistance allele into Neisseria meningitidis.Microbial genomics · 2018Article
- Evolution via recombination: Cell-to-cell contact facilitates larger recombination events in Streptococcus pneumoniae.PLoS genetics · 2018Article
- Coalescent framework for prokaryotes undergoing interspecific homologous recombination.Heredity · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Helicobacter pylori are gram-negative bacteria notable for their high level of genetic diversity and plasticity, features that may play a key role in the organism's ability to colonize the human stomach. Homeologous natural transformation, a key contributor to genomic diversification, has been well-described for H. pylori. To examine the mechanisms involved, we performed restriction analysis and sequencing of recombination products to characterize the length, fragmentation, and position of DNA imported via natural transformation. Our analysis revealed DNA imports of small size (1,300 bp, 95% confidence limits 950-1850 bp) with instances of substantial asymmetry in relation to selectable antibiotic-resistance markers. We also observed clustering of imported DNA endpoints, suggesting a possible role for restriction endonucleases in limiting recombination length. Additionally, we observed gaps in integrated DNA and found evidence suggesting that these gaps are the result of two or more separate strand invasions. Taken together, these observations support a system of highly efficient short-fragment recombination involving multiple recombination events within a single locus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.