ArticleNature protocols2009
Measurement of auxin transport in Arabidopsis thaliana.
Article in Nature protocols, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
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Who cites it
67 citing papers in PubMed.
- Quantitative Measurement of Basipetal Auxin Transport in Arabidopsis Roots ViaMethods in molecular biology (Clifton, N.J.) · 2027Article
- HSP90 differentially stabilizes plant ABCB-type auxin transporters on the plasma membrane.Nature communications · 2025Article
- An experimental approach to study foraging memory in ectomycorrhizal mycelium.Communicative & integrative biology · 2025Article
- Nonspecific phospholipases C3 and C4 interact with PIN-FORMED2 to regulate growth and tropic responses in Arabidopsis.The Plant cell · 2024Article
- Divergence of trafficking and polarization mechanisms for PIN auxin transporters during land plant evolution.Plant communications · 2024Article
- ABCB-mediated shootward auxin transport feeds into the root clock.EMBO reports · 2023Article
- WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions.Nature communications · 2022Article
- KAI2 regulates seedling development by mediating light-induced remodelling of auxin transport.The New phytologist · 2022Article
- Increased branching independent of strigolactone in cytokinin oxidase 2-overexpressing tomato is mediated by reduced auxin transport.Molecular horticulture · 2022Article
- Screening of protonstatin-1 (PS-1) analogs for improved inhibitors of plant plasma membrane HFrontiers in plant science · 2022Article
- The role of auxin and sugar signaling in dominance inhibition of inflorescence growth by fruit load.Plant physiology · 2021Article
- GmPIN-dependent polar auxin transport is involved in soybean nodule development.The Plant cell · 2021Article
- Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport.The EMBO journal · 2021Article
- Flavonol-mediated stabilization of PIN efflux complexes regulates polar auxin transport.The EMBO journal · 2021Article
- The Photosynthetic BacteriumFrontiers in plant science · 2021Article
- Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana.The New phytologist · 2021Article
- The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes.Plant communications · 2020Article
- OsNAR2.1 Interaction with OsNIT1 and OsNIT2 Functions in Root-growth Responses to Nitrate and Ammonium.Plant physiology · 2020Article
- The lipid code-dependent phosphoswitch PDK1-D6PK activates PIN-mediated auxin efflux in Arabidopsis.Nature plants · 2020Article
- Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters.The Plant cell · 2020Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This protocol allows the measurement of auxin transport in roots, hypocotyls and inflorescences of Arabidopsis thaliana plants by examining transport of radiolabeled auxin or movement of an auxin-induced gene expression signal. The protocol contains four stages: seedling growth, auxin application, a transport period of variable length, and quantification of auxin movement or reporter expression. Beyond the time for plant growth, the transport assay can be completed within 4-18 h. Auxin is applied to seedlings in agar cylinders or droplets, which does not require specialized liquid-handling equipment or micromanipulators, in contrast with methods that apply auxin in liquid droplets. Spatial control of auxin application is reduced, but this method has the advantages of being technically more feasible for most laboratories and allowing agar containing radioactive auxin to be removed for pulse chase assays that determine transport rates. These methods allow investigation of genetic and environmental factors that control auxin transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.