ArticleNucleic acids research2009
Novel method for high-throughput colony PCR screening in nanoliter-reactors.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Study on Cloning and Expression of TNF-α Variants inProtein and peptide letters · 2024Article
- Ultrahigh-Throughput Enzyme Engineering and Discovery inChemical reviews · 2023Review
- "Basicles": Microbial Growth and Production Monitoring in Giant Lipid Vesicles.ACS applied materials & interfaces · 2019Article
- Analysis of modular bioengineered antimicrobial lanthipeptides at nanoliter scale.Nature chemical biology · 2019Article
- Archimedes' principle for characterisation of recombinant whole cell biocatalysts.Scientific reports · 2018Article
- Finding a helix in a haystack: nucleic acid cytometry with droplet microfluidics.Lab on a chip · 2017Review
- Optimization of a whole-cell biocatalyst by employing genetically encoded product sensors inside nanolitre reactors.Nature chemistry · 2015Article
- A simple method for encapsulating single cells in alginate microspheres allows for direct PCR and whole genome amplification.PloS one · 2015Article
- Single-cell multiplex gene detection and sequencing with microfluidically generated agarose emulsions.Angewandte Chemie (International ed. in English) · 2011Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We introduce a technology for the rapid identification and sequencing of conserved DNA elements employing a novel suspension array based on nanoliter (nl)-reactors made from alginate. The reactors have a volume of 35 nl and serve as reaction compartments during monoseptic growth of microbial library clones, colony lysis, thermocycling and screening for sequence motifs via semi-quantitative fluorescence analyses. nl-Reactors were kept in suspension during all high-throughput steps which allowed performing the protocol in a highly space-effective fashion and at negligible expenses of consumables and reagents. As a first application, 11 high-quality microsatellites for polymorphism studies in cassava were isolated and sequenced out of a library of 20,000 clones in 2 days. The technology is widely scalable and we envision that throughputs for nl-reactor based screenings can be increased up to 100,000 and more samples per day thereby efficiently complementing protocols based on established deep-sequencing technologies.
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Registered trials
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