ArticleThe Journal of biological chemistry2009
Molecular basis for class V beta-tubulin effects on microtubule assembly and paclitaxel resistance.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- β3 accelerates microtubule plus end maturation through a divergent lateral interface.Molecular biology of the cell · 2025Article
- SLC15A3 is transcriptionally regulated by HIF1α and p65 to worsen neuroinflammation in experimental ischemic stroke.Molecular neurobiology · 2024Article
- β3 accelerates microtubule plus end maturation through a divergent lateral interface.bioRxiv : the preprint server for biology · 2024Article
- Review
- Combining MEK and SRC inhibitors for treatment of colorectal cancer demonstrate increased efficacy in vitro but not in vivo.PloS one · 2023Article
- Nuclear βII-Tubulin and its Possible Utility in Cancer Diagnosis, Prognosis and Treatment.Frontiers in cell and developmental biology · 2022Article
- Possible Roles of Specific Amino Acids in β-Tubulin Isotypes in the Growth and Maintenance of Neurons: Novel Insights From Cephalopod Mollusks.Frontiers in molecular neuroscience · 2022Article
- The Beta-Tubulin Isotype TUBB6 Controls Microtubule and Actin Dynamics in Osteoclasts.Frontiers in cell and developmental biology · 2021Article
- Persistent upregulation of the β-tubulin tubb6, linked to muscle regeneration, is a source of microtubule disorganization in dystrophic muscle.Human molecular genetics · 2019Article
- Paclitaxel Through the Ages of Anticancer Therapy: Exploring Its Role in Chemoresistance and Radiation Therapy.Cancers · 2015Review
- Prognostic biomarkers for HNSCC using quantitative real-time PCR and microarray analysis: β-tubulin isotypes and the p53 interactome.Cytoskeleton (Hoboken, N.J.) · 2014Article
- Proteomics of cancer cell lines resistant to microtubule-stabilizing agents.Molecular cancer therapeutics · 2014Article
- A cellular genome-wide association study reveals human variation in microtubule stability and a role in inflammatory cell death.Molecular biology of the cell · 2014Article
- Random mutagenesis of β-tubulin defines a set of dispersed mutations that confer paclitaxel resistance.Pharmaceutical research · 2012Article
- βV-tubulin expression is associated with outcome following taxane-based chemotherapy in non-small cell lung cancer.British journal of cancer · 2012Article
- Characterization of a human βV-tubulin antibody and expression of this isotype in normal and malignant human tissue.Cytoskeleton (Hoboken, N.J.) · 2012Article
- Distinct functional roles of β-tubulin isotypes in microtubule arrays of Tetrahymena thermophila, a model single-celled organism.PloS one · 2012Article
- New insights into mechanisms of resistance to microtubule inhibitors.Biochimica et biophysica acta · 2011Review
- Class V β-tubulin alters dynamic instability and stimulates microtubule detachment from centrosomes.Molecular biology of the cell · 2011Article
- Microtubule-binding agents: a dynamic field of cancer therapeutics.Nature reviews. Drug discovery · 2010Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Vertebrates produce at least seven distinct beta-tubulin isotypes that coassemble into all cellular microtubules. The functional differences among these tubulin isoforms are largely unknown, but recent studies indicate that tubulin composition can affect microtubule properties and cellular microtubule-dependent behavior. One of the isotypes whose incorporation causes the largest change in microtubule assembly is beta5-tubulin. Overexpression of this isotype can almost completely destroy the microtubule network, yet it appears to be required in smaller amounts for normal mitotic progression. Moderate levels of overexpression can also confer paclitaxel resistance. Experiments using chimeric constructs and site-directed mutagenesis now indicate that the hypervariable C-terminal region of beta5 plays no role in these phenotypes. Instead, we demonstrate that two residues found in beta5 (Ser-239 and Ser-365) are each sufficient to inhibit microtubule assembly and confer paclitaxel resistance when introduced into beta1-tubulin; yet the single mutation of residue Ser-239 in beta5 eliminates its ability to confer these phenotypes. Despite the high degree of conservation among beta-tubulin isotypes, mutations affecting residue 365 demonstrate that amino acid substitutions can be context sensitive; i.e. an amino acid change in one isotype will not necessarily produce the same phenotype when introduced into a different isotype. Modeling studies indicate that residue Cys-239 of beta1-tubulin is close to a highly conserved Cys-354 residue suggesting the possibility that disulfide formation could play a significant role in the stability of microtubules formed with beta1- but not with beta5-tubulin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.