ArticleDifferentiation; research in biological diversity2009
Differentiation-dependent modification and subcellular distribution of aquaporin-0 suggests multiple functional roles in the rat lens.
Article in Differentiation; research in biological diversity, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 38 citations in OpenAlex.
- CatExperimental eye research · 2026Article
- Verification of the gene and protein expression of the aquaglyceroporin AQP3 in the mammalian lens.Experimental eye research · 2024Article
- Tissue, cellular, and molecular level determinants for eye lens stiffness and elasticity.Frontiers in ophthalmology · 2024Review
- Native Ambient Mass Spectrometry of an Intact Membrane Protein Assembly and Soluble Protein Assemblies Directly from Lens Tissue.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2022Article
- Native Ambient Mass Spectrometry of an Intact Membrane Protein Assembly and Soluble Protein Assemblies Directly from Lens Tissue.Angewandte Chemie (International ed. in English) · 2022Article
- Beyond the Channels: Adhesion Functions of Aquaporin 0 and Connexin 50 in Lens Development.Frontiers in cell and developmental biology · 2022Review
- Lens Aquaporins in Health and Disease: Location is Everything!Frontiers in physiology · 2022Review
- Spatially Resolved Proteomic Analysis of the Lens Extracellular Diffusion Barrier.Investigative ophthalmology & visual science · 2021Article
- Aquaporins Have Regional Functions in Development of Refractive Index in the Zebrafish Eye Lens.Investigative ophthalmology & visual science · 2021Article
- Eph-ephrin Signaling Affects Eye Lens Fiber Cell Intracellular Voltage and Membrane Conductance.Frontiers in physiology · 2021Article
- Localization of the lens intermediate filament switch by imaging mass spectrometry.Experimental eye research · 2020Article
- Changes to Zonular Tension Alters the Subcellular Distribution of AQP5 in Regions of Influx and Efflux of Water in the Rat Lens.Investigative ophthalmology & visual science · 2020Article
- Connexin 50 and AQP0 are Essential in Maintaining Organization and Integrity of Lens Fibers.Investigative ophthalmology & visual science · 2019Article
- Aqp0a Regulates Suture Stability in the Zebrafish Lens.Investigative ophthalmology & visual science · 2018Article
- Dynamic functional contribution of the water channel AQP5 to the water permeability of peripheral lens fiber cells.American journal of physiology. Cell physiology · 2018Article
- The Role of Aquaporins in Ocular Lens Homeostasis.International journal of molecular sciences · 2017Review
- Identification of a direct Aquaporin-0 binding site in the lens-specific cytoskeletal protein filensin.Experimental eye research · 2017Article
- Spatial distributions of phosphorylated membrane proteins aquaporin 0 and MP20 across young and aged human lenses.Experimental eye research · 2016Article
- MALDI Imaging Mass Spectrometry Spatially Maps Age-Related Deamidation and Truncation of Human Lens Aquaporin-0.Investigative ophthalmology & visual science · 2015Article
- Spatial distributions of AQP5 and AQP0 in embryonic and postnatal mouse lens development.Experimental eye research · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Using immunohistochemistry and mass spectrometry, differentiation-dependent changes in the subcellular distribution and processing of aquaporin-0 (AQP0) have been mapped in the rat lens. Sections labelled with C-terminal tail AQP0 antibodies yielded two concentric rings of labelling with minimal signal in the lens core. The rings were separated by a transient zone of decreased labelling located prior to the transition of differentiating fiber (DF) cells into mature denucleated fiber (MF) cells. Mass spectrometry showed that the loss of core labelling was due to AQP0 cleavage, while the transient loss of labelling was more likely caused by masking of the antibody epitope. AQP0 subcellular distribution changed with radial distance into the lens. In peripheral DF cells, AQP0 was found throughout both broad and narrow side membranes. In deeper-lying DF cells, AQP0 aggregated into plaque-like structures located on the broad sides. This shift occurred prior to the transient loss of AQP0 signal, and coincided with formation of broad-side membrane invaginations between adjacent fiber cells to which filensin, a known binding partner of AQP0, was also localized. After nuclei loss, AQP0 was once again distributed throughout MF cell membranes. In the absence of protein synthesis, the observed subcellular redistribution of AQP0 in DF and subsequent cleavage of AQP0 in MF are suggestive of a switch in the function of AQP0 from a water channel to a junctional protein.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.