ArticleLangmuir : the ACS journal of surfaces and colloids2009
Controlling oligonucleotide surface density in light-directed DNA array fabrication.
Article in Langmuir : the ACS journal of surfaces and colloids, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Efficiency of Digital Photolithographic Synthesis of Large, High-Quality DNA Libraries and Microarrays using a Guanine OCommunications chemistry · 2025Article
- Sequence Preference and Initiator Promiscuity forACS synthetic biology · 2021Article
- High-Efficiency Reverse (5'→3') Synthesis of Complex DNA Microarrays.Scientific reports · 2018Article
- Photolithographic Synthesis of High-Density DNA and RNA Arrays on Flexible, Transparent, and Easily Subdivided Plastic Substrates.Analytical chemistry · 2015Article
- Electrochemistry of nonconjugated proteins and glycoproteins. Toward sensors for biomedicine and glycomics.Chemical reviews · 2015Review
- Carbon Substrates: A Stable Foundation for Biomolecular Arrays.Annual review of analytical chemistry (Palo Alto, Calif.) · 2015Review
- Enzymatic fabrication of high-density RNA arrays.Angewandte Chemie (International ed. in English) · 2014Article
- Optimized light-directed synthesis of aptamer microarrays.Analytical chemistry · 2013Article
- TATA binding proteins can recognize nontraditional DNA sequences.Biophysical journal · 2012Article
- Efficiency, error and yield in light-directed maskless synthesis of DNA microarrays.Journal of nanobiotechnology · 2011Article
- Accurate prediction of binding thermodynamics for DNA on surfaces.The journal of physical chemistry. B · 2011Article
- Article
- Photopatterned thiol surfaces for biomolecule immobilization.Langmuir : the ACS journal of surfaces and colloids · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Over the past two decades high-density DNA arrays have developed into a central technology for nucleic acid analyses. Important application areas include whole-genome gene expression studies, high throughput analyses of single nucleotide polymorphisms, and, most recently, the determination of binding site specificities for transcription factors and other critical elements involved in gene regulation. A key parameter in the performance of DNA arrays is the density of the surface-bound oligonucleotides, which strongly affects both thermodynamic and kinetic aspects of DNA hybridization. In this report, we describe an approach for the control of oligonucleotide density in photolithographically fabricated DNA arrays, based upon a controlled UV light deprotection procedure. Modulation of the UV exposure permits a desired degree of deprotection of surface synthesis sites; a subsequent capping reaction to inactivate the exposed sites leaves only a desired fraction of active sites remaining for synthesis, corresponding to a lower oligonucleotide density. It is shown that the procedure is reasonably general, in that it is readily transferable to alternative substrate materials with similar results.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.