ArticlePlant physiology2009
System analysis of an Arabidopsis mutant altered in de novo fatty acid synthesis reveals diverse changes in seed composition and metabolism.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 77 citations in OpenAlex.
- GWAS-based identification of GmKASII and its interaction with GmFATA1B reveals their role in soybean oil composition and accumulation.The Plant journal : for cell and molecular biology · 2026Article
- Proximity labelling suggests association of the nonhost receptor PSS1 protein with enzymes of multiple defense pathways in Arabidopsis.Frontiers in plant science · 2025Article
- Evolution of the regulatory subunits for the heteromeric acetyl-CoA carboxylase.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Bioinformatics Identification and Expression Analysis of Acetyl-CoA Carboxylase Reveal Its Role in Isoflavone Accumulation during Soybean Seed Development.International journal of molecular sciences · 2024Article
- Proteomic profile of the germinating seeds reveals enhanced seedling growth in Arabidopsis rpp1a mutant.Plant molecular biology · 2023Article
- Integrative Metabolic and Transcriptomic Profiling inPlants (Basel, Switzerland) · 2023Article
- The Sapria himalayana genome provides new insights into the lifestyle of endoparasitic plants.BMC biology · 2023Article
- Genome-Wide Identification and Expression Profiling ofFrontiers in plant science · 2022Article
- Overexpression of Type 1 and 2 Diacylglycerol Acyltransferase Genes (Plants (Basel, Switzerland) · 2021Article
- Effects of type I Diacylglycerol O-acyltransferase (DGAT1) genes on soybean (Glycine max L.) seed composition.Scientific reports · 2021Article
- The BADC and BCCP subunits of chloroplast acetyl-CoA carboxylase sense the pH changes of the light-dark cycle.The Journal of biological chemistry · 2020Article
- Non-Catalytic Subunits Facilitate Quaternary Organization of Plastidic Acetyl-CoA Carboxylase.Plant physiology · 2020Article
- Article
- Design of a Seed-Specific Chimeric Promoter with a Modified Expression Profile to Improve Seed Oil Content.International journal of molecular sciences · 2018Article
- Transcriptomic and Metabolomic Reprogramming from Roots to Haustoria in the Parasitic Plant, Thesium chinense.Plant & cell physiology · 2018Article
- Differential gene expression at different stages of mesocarp development in high- and low-yielding oil palm.BMC genomics · 2017Article
- An Induced Chromosomal Translocation in Soybean Disrupts aG3 (Bethesda, Md.) · 2017Article
- A Family of Negative Regulators Targets the Committed Step of de Novo Fatty Acid Biosynthesis.The Plant cell · 2016Article
- An Integrated Bioinformatics Analysis Reveals Divergent Evolutionary Pattern of Oil Biosynthesis in High- and Low-Oil Plants.PloS one · 2016Article
- Chlorophyll and carbohydrate metabolism in developing silique and seed are prerequisite to seed oil content of Brassica napus L.Botanical studies · 2014Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryo-specific overexpression of biotin carboxyl carrier protein 2 (BCCP2) inhibited plastid acetyl-coenzyme A carboxylase (ACCase), resulting in altered oil, protein, and carbohydrate composition in mature Arabidopsis (Arabidopsis thaliana) seed. To characterize gene and protein regulatory consequences of this mutation, global microarray, two-dimensional difference gel electrophoresis, iTRAQ, and quantitative immunoblotting were performed in parallel. These analyses revealed that (1) transgenic overexpression of BCCP2 did not affect the expression of three other ACCase subunits; (2) four subunits to plastid pyruvate dehydrogenase complex were 25% to 70% down-regulated at protein but not transcript levels; (3) key glycolysis and de novo fatty acid/lipid synthesis enzymes were induced; (4) multiple storage proteins, but not cognate transcripts, were up-regulated; and (5) the biotin synthesis pathway was up-regulated at both transcript and protein levels. Biotin production appears closely matched to endogenous BCCP levels, since overexpression of BCCP2 produced mostly apo-BCCP2 and the resulting ACCase-compromised, low-oil phenotype. Differential expression of glycolysis, plastid pyruvate dehydrogenase complex, fatty acid, and lipid synthesis activities indicate multiple, complex regulatory responses including feedback as well as futile "feed-forward" elicitation in the case of fatty acid and lipid biosynthetic enzymes. Induction of storage proteins reveals that oil and protein synthesis share carbon intermediate(s) and that reducing malonyl-coenzyme A flow into fatty acids diverts carbon into amino acid and protein synthesis.
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