ArticleNucleic acids research2009
A recurrent magnesium-binding motif provides a framework for the ribosomal peptidyl transferase center.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 71 citations in OpenAlex.
- The master molecule that built biology: How water shaped the chemistry of life.Protein science : a publication of the Protein Society · 2026Review
- RNA-Iron complexes catalyse prebiotic oxygen generation.Communications chemistry · 2026Article
- Cat_Wiz: a stereochemistry-guided toolkit for locating, diagnosing, and annotating Mg2+ ions in RNA structures.Nucleic acids research · 2026Article
- Principles of ion binding to RNA inferred from the analysis of a 1.55 Å resolution bacterial ribosome structure - Part I: Mg2.Nucleic acids research · 2025Article
- Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling.International journal of molecular sciences · 2023Article
- The Peptidyl Transferase Center: a Window to the Past.Microbiology and molecular biology reviews : MMBR · 2021Article
- Interchangeable utilization of metals: New perspectives on the impacts of metal ions employed in ancient and extant biomolecules.The Journal of biological chemistry · 2021Review
- An uncommon [KRNA (New York, N.Y.) · 2021Article
- smFRET study of rRNA dimerization at the peptidyl transfer center.Biophysical chemistry · 2021Article
- Further Characterization of the Pseudo-Symmetrical Ribosomal Region.Life (Basel, Switzerland) · 2020Article
- Cutting in-line with iron: ribosomal function and non-oxidative RNA cleavage.Nucleic acids research · 2020Article
- Piece by piece: Building a ribozyme.The Journal of biological chemistry · 2020Review
- Prebiotic Iron Originates the Peptidyl Transfer Origin.Molecular biology and evolution · 2019Article
- Nucleobase carbonyl groups are poor MgRNA (New York, N.Y.) · 2019Article
- Multiple prebiotic metals mediate translation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Green Rust: The Simple Organizing 'Seed' of All Life?Life (Basel, Switzerland) · 2018Review
- RNA Structural Dynamics As Captured by Molecular Simulations: A Comprehensive Overview.Chemical reviews · 2018Review
- Visualization of conformational variability in the domains of long single-stranded RNA molecules.Nucleic acids research · 2017Article
- Iron mediates catalysis of nucleic acid processing enzymes: support for Fe(II) as a cofactor before the great oxidation event.Nucleic acids research · 2017Article
- The ribosome challenge to the RNA world.Journal of molecular evolution · 2015Review
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2 authors at 1 institution in 1 country.
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Abstract
The ribosome is an ancient macromolecular machine responsible for the synthesis of all proteins in all living organisms. Here we demonstrate that the ribosomal peptidyl transferase center (PTC) is supported by a framework of magnesium microclusters (Mg(2+)-muc's). Common features of Mg(2+)-muc's include two paired Mg(2+) ions that are chelated by a common bridging phosphate group in the form Mg((a))(2+)-(O1P-P-O2P)-Mg((b))(2+). This bridging phosphate is part of a 10-membered chelation ring in the form Mg((a))(2+)-(OP-P-O5'-C5'-C4'-C3'-O3'-P-OP)-Mg((a))(2+). The two phosphate groups of this 10-membered ring are contributed by adjacent residues along the RNA backbone. Both Mg(2+) ions are octahedrally coordinated, but are substantially dehydrated by interactions with additional RNA phosphate groups. The Mg(2+)-muc's in the LSU (large subunit) appear to be highly conserved over evolution, since they are unchanged in bacteria (Thermus thermophilus, PDB entry 2J01) and archaea (Haloarcula marismortui, PDB entry 1JJ2). The 2D elements of the 23S rRNA that are linked by Mg(2+)-muc's are conserved between the rRNAs of bacteria, archaea and eukarya and in mitochondrial rRNA, and in a proposed minimal 23S-rRNA. We observe Mg(2+)-muc's in other rRNAs including the bacterial 16S rRNA, and the P4-P6 domain of the tetrahymena Group I intron ribozyme. It appears that Mg(2+)-muc's are a primeval motif, with pivotal roles in RNA folding, function and evolution.
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