ArticleNucleic acids research2009
Efficient mouse transgenesis using Gateway-compatible ROSA26 locus targeting vectors and F1 hybrid ES cells.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 149 citations in OpenAlex.
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- Limitations of human tau-expressing mouse models and novel approaches of mouse modeling for tauopathy.Frontiers in neuroscience · 2023Review
- Targeted Integration of Transgenes at the Mouse Gt(ROSA)26Sor Locus.Methods in molecular biology (Clifton, N.J.) · 2023Article
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- Gasdermin-B Pro-Tumor Function in Novel Knock-in Mouse Models Depends on theFrontiers in cell and developmental biology · 2022Article
- Ret signaling in ureteric bud epithelial cells controls cell movements, cell clustering and bud formation.Development (Cambridge, England) · 2021Article
- A New Transgenic Mouse Line for Imaging Mitochondrial Calcium Signals.Function (Oxford, England) · 2021Article
- Article
- PI 3-kinase delta enhances axonal PIPEMBO molecular medicine · 2020Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
18 authors at 4 institutions in 3 countries.
Funding
Abstract
The ability to rapidly and efficiently generate reliable Cre/loxP conditional transgenic mice would greatly complement global high-throughput gene targeting initiatives aimed at identifying gene function in the mouse. We report here the generation of Cre/loxP conditional ROSA26-targeted ES cells within 3-4 weeks by using Gateway cloning to build the target vectors. The cDNA of the gene of interest can be expressed either directly by the ROSA26 promoter providing a moderate level of expression or by a CAGG promoter placed in the ROSA26 locus providing higher transgene expression. Utilization of F1 hybrid ES cells with exceptional developmental potential allows the production of germ line transmitting, fully or highly ES cell-derived mice by aggregation of cells with diploid embryos. The presented streamlined procedures accelerate the examination of phenotypical consequences of transgene expression. It also provides a unique tool for comparing the biological activity of polymorphic or splice variants of a gene, or products of different genes functioning in the same or parallel pathways in an overlapping manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.