ArticleJournal of clinical microbiology2009
High levels of mecA DNA detected by a quantitative real-time PCR assay are associated with mortality in patients with methicillin-resistant Staphylococcus aureus bacteremia.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Rapid molecular diagnostic tests in patients with bacteremia: evaluation of their impact on decision making and clinical outcomes.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Pooled it
- Microbubble-Controlled Delivery of Biofilm-Targeting Nanoparticles to Treat MRSA Infection.Advanced functional materials · 2025Article
- Clinical diagnostic performance of droplet digital PCR for pathogen detection in patients with Escherichia coli bloodstream infection: a prospective observational study.BMC infectious diseases · 2025Observational
- Damage-associated molecular patterns in bacteraemic infection, including a comparative analysis with bacterial DNA, a pathogen-associated molecular pattern.Scientific reports · 2024Article
- Estimating bacterial load in S. aureus and E. coli bacteremia using bacterial growth graph from the continuous monitoring blood culture system.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Prognostic value of poly-microorganisms detected by droplet digital PCR and pathogen load kinetics in sepsis patients: a multi-center prospective cohort study.Microbiology spectrum · 2024Article
- Direct-from-Blood Detection of Pathogens: a Review of Technology and Challenges.Journal of clinical microbiology · 2023Review
- Pathogen load and species monitored by droplet digital PCR in patients with bloodstream infections: A prospective case series study.BMC infectious diseases · 2022Article
- Bacterial DNA load in Staphylococcus aureus bacteremia is significantly higher in intravascular infections.PloS one · 2022Article
- Sustained Circulating Bacterial Deoxyribonucleic Acid Is Associated With ComplicatedOpen forum infectious diseases · 2019Article
- 16S rDNA droplet digital PCR for monitoring bacterial DNAemia in bloodstream infections.PloS one · 2019Article
- Clinical Usefulness of Real-Time Polymerase Chain Reaction for the Diagnosis ofThe American journal of tropical medicine and hygiene · 2017Article
- Self-powered integrated microfluidic point-of-care low-cost enabling (SIMPLE) chip.Science advances · 2017Article
- Malaria real-time PCR: correlation with clinical presentation.New microbes and new infections · 2015Article
- Rapid Treponema pallidum clearance from blood and ulcer samples following single dose benzathine penicillin treatment of early syphilis.PLoS neglected tropical diseases · 2015Article
- Improved sensitivity for molecular detection of bacterial and Candida infections in blood.Journal of clinical microbiology · 2014Article
- Quantitative data from the SeptiFast real-time PCR is associated with disease severity in patients with sepsis.BMC infectious diseases · 2014Article
- The meta-genome of sepsis: host genetics, pathogens and the acute immune response.Journal of innate immunity · 2014Review
- The complex pathogenesis of bacteremia: from antimicrobial clearance mechanisms to the genetic background of the host.Virulence · 2014Review
- The potential for PCR based testing to improve diagnosis and treatment of sepsis.Current infectious disease reports · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Persistent methicillin-resistant Staphylococcus aureus (MRSA) bacteremia is known to be a poor prognostic factor. While several PCR assays for the detection of MRSA in various clinical samples were recently reported, the possibility that a quantitative PCR assay could be used to quantify and monitor MRSA bacteremia has not been explored. In this study, we established a quantitative real-time PCR assay for the mecA gene using known copy numbers of a plasmid containing mecA DNA as a standard and the previously described mecA-specific primers and probe (P. Francois et al., J. Clin. Microbiol. 41:254-260, 2003). We employed this assay to examine 250 sequential whole-blood samples from 20 adult patients, including 13 survivors and 7 nonsurvivors, with culture-proven MRSA bacteremia at the intensive care units of National Taiwan University Hospital between 1 July 2006 and 31 January 2007. The levels of mecA DNA in the nonsurvivors were significantly higher than those in the survivors during the three periods of bacteremia examined (days 0 to 2, 3 to 5, and 6 to 8) (P = 0.003 by two-tailed Mann-Whitney U test). Moreover, the nonsurvivors had higher mecA DNA levels than the survivors after 3 days and 7 days of anti-MRSA therapy (medians for nonsurvivors and survivors at 3 days, 5.86 and 4.30 log copies/ml, respectively; medians for nonsurvivors and survivors at 7 days, 5.21 and 4.36 log copies/ml, respectively; P = 0.02 and P = 0.04, respectively, by two-tailed Mann-Whitney U test). Together, these findings suggest that the level of mecA DNA in blood could potentially be used to monitor MRSA bacteremia and evaluate responses to therapy.
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