ArticleJournal of neurophysiology2009
Stimulation-induced formation of the reserve pool of vesicles in Drosophila motor boutons.
Article in Journal of neurophysiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Developmental and physiological impacts of pathogenic human huntingtin protein in the nervous system.Neurobiology of disease · 2024Article
- Minibrain kinase and calcineurin coordinate activity-dependent bulk endocytosis through synaptojanin.The Journal of cell biology · 2021Article
- Article
- Nitric oxide-mediated posttranslational modifications control neurotransmitter release by modulating complexin farnesylation and enhancing its clamping ability.PLoS biology · 2018Article
- Article
- Limited distal organelles and synaptic function in extensive monoaminergic innervation.Journal of cell science · 2017Article
- Complexin Mutants Reveal Partial Segregation between Recycling Pathways That Drive Evoked and Spontaneous Neurotransmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Interaction of the Complexin Accessory Helix with Synaptobrevin Regulates Spontaneous Fusion.Biophysical journal · 2016Article
- Phosphorylation of Synaptojanin Differentially Regulates Endocytosis of Functionally Distinct Synaptic Vesicle Pools.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- An Endocytic Scaffolding Protein together with Synapsin Regulates Synaptic Vesicle Clustering in the Drosophila Neuromuscular Junction.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Disruption of Endocytosis with the Dynamin Mutant shibirets1 Suppresses Seizures in Drosophila.Genetics · 2015Article
- Article
- Interaction of the complexin accessory helix with the C-terminus of the SNARE complex: molecular-dynamics model of the fusion clamp.Biophysical journal · 2013Article
- Adhesion energy can regulate vesicle fusion and stabilize partially fused states.Journal of the Royal Society, Interface · 2012Article
- Recruitment of resting vesicles into recycling pools supports NMDA receptor-dependent synaptic potentiation in cultured hippocampal neurons.The Journal of physiology · 2012Article
- Synapsin regulates vesicle organization and activity-dependent recycling at Drosophila motor boutons.Neuroscience · 2010Article
- Nonmuscle Myosin II helps regulate synaptic vesicle mobility at the Drosophila neuromuscular junction.BMC neuroscience · 2010Article
- Synaptic vesicle pools: an update.Frontiers in synaptic neuroscience · 2010Article
- The molecular physiology of activity-dependent bulk endocytosis of synaptic vesicles.Journal of neurochemistry · 2009Review
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Authors and funding
2 authors.
Funding
Abstract
We combined electron microscopy (EM), synaptic vesicle staining by fluorescent marker FM1-43, photoconversion of the dye into an electron dense product, and electrical recordings of synaptic responses to study the distribution of reserve and recycling vesicles and its dependence on stimulation in Drosophila motor boutons. We showed that, at rest, vesicles are distributed over the periphery of the bouton, with the recycling and reserve pools being intermixed and the central core of the bouton being devoid of vesicles. Continuous high-frequency stimulation followed by a resting period mobilized the reserve vesicles into the recycling pool and, most notably, produced an increase in vesicle abundance. Recordings of synaptic activity from the temperature-sensitive endocytosis mutant shibire during continuous stimulation until complete depression provided an independent estimate of the increase in vesicle abundance on intense stimulation. EM analysis demonstrated that continuous stimulation produced an increase in the vesicle density, whereas during a subsequent resting period, vesicles filled empty areas of the bouton, spreading toward its central core. Although the observed structural potentiation did not alter basal transmitter release, it produced an increased synaptic enhancement during high-frequency stimulation. The latter effect was not observed when the boutons were potentiated using high-frequency stimulation without a subsequent resting period. We concluded therefore that the newly formed vesicles replenish the reserve pool during a resting period following intense stimulation.
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