ArticleThe Journal of biological chemistry2009
GATA transcription factors regulate the expression of the human eosinophil-derived neurotoxin (RNase 2) gene.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Discovery of Innate Immune Response mRNAs That Are Impacted by Structure-Specific Oral Baker's Yeast Beta Glucan Consumption.Biotech (Basel (Switzerland)) · 2025Article
- Biological Activities of Secretory RNases: Focus on Their Oligomerization to Design Antitumor Drugs.Frontiers in immunology · 2019Review
- The Immunomodulatory and Antimicrobial Properties of the Vertebrate Ribonuclease A Superfamily.Vaccines · 2018Review
- A CREB-mediated increase in miRNA let-7f during prolonged β-agonist exposure: a novel mechanism of βFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Bexarotene inhibits the viability of non-small cell lung cancer cells via slc10a2/PPARγ/PTEN/mTOR signaling pathway.BMC cancer · 2018Article
- RhoH is a negative regulator of eosinophilopoiesis.Cell death and differentiation · 2016Article
- Human Th17 Cells Lack HIV-Inhibitory RNases and Are Highly Permissive to Productive HIV Infection.Journal of virology · 2016Article
- Simvastatin Inhibits IL-5-Induced Chemotaxis and CCR3 Expression of HL-60-Derived and Human Primary Eosinophils.PloS one · 2016Article
- CpG methylation at GATA elements in the regulatory region of CCR3 positively correlates with CCR3 transcription.Experimental & molecular medicine · 2012Article
- Eosinophil development, regulation of eosinophil-specific genes, and role of eosinophils in the pathogenesis of asthma.Allergy, asthma & immunology research · 2012Article
- Article
- The gene structure and promoter region of the vaccine target aminopeptidase H11 from the blood-sucking nematode parasite of ruminants, Haemonchus contortus.Functional & integrative genomics · 2010Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The transcription factors GATA-1 and GATA-2 have been implicated in promoting differentiation of eosinophilic leukocytes. In this study, we examined the roles of GATA-1 and GATA-2 in activating transcription of the secretory ribonuclease, the eosinophil-derived neurotoxin (EDN/RNase 2). Augmented expression of both GATA-1 and GATA-2 was detected in eosinophil promyelocyte HL-60 clone 15 cells in response to biochemical differentiation with butyric acid. Deletion or mutation of one or both of the two consensus GATA-binding sites in the extended 1000-bp 5' promoter of the EDN gene resulted in profound reduction in reporter gene activity. Antibody-augmented electrophoretic mobility shift and chromatin immunoprecipitation analyses indicate that GATA-1 and GATA-2 proteins bind to both functional GATA consensus sequences in the EDN promoter. Interestingly, RNA silencing of GATA-1 alone had no impact on EDN expression; silencing of GATA-2 resulted in diminished expression of EDN, and also diminished expression of GATA-1 in both butyric acid-induced HL-60 clone 15 cells and in differentiating human eosinophils derived from CD34(+) hematopoietic progenitors. Likewise, overexpression of GATA-2 in uninduced HL-60 clone 15 cells resulted in augmented transcription of both EDN and GATA-1. Taken together, our data suggest that GATA-2 functions directly via interactions with the EDN promoter and also indirectly, via its ability to regulate the expression of GATA-1 in differentiating eosinophils and eosinophil cell lines.
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