ArticleThe Journal of biological chemistry2009
Cell wall beta-(1,6)-glucan of Saccharomyces cerevisiae: structural characterization and in situ synthesis.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 145 citations in OpenAlex.
- A bipartite glucan synthase-remodeler module organizes branched glucan assembly in the fungal cell wall.Nature communications · 2026Article
- The Structure, Biosynthesis, and Function of β-1,6-Glucan in the Fungal Cell Wall.Biomolecules · 2026Review
- How Complex Dietary Fibers Can Be Used to Shape the Human Gut Microbiome Toward Reduced Inflammatory Potential: A Pilot Study.Molecules (Basel, Switzerland) · 2026Article
- Structural Characterization and Immunostimulatory Activity of a New Slightly Branched β-glucan in Sporisorium reilianum-Infected Sorghum Head Smut.Chemistry & biodiversity · 2026Article
- Aspergillus fumigatus Cell Wall: Current Advances in Structural Elucidation Methods.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Sustainable carbon quantum dots synthesized from yeast β-glucan as a promising nanomaterial for biological applications.Scientific reports · 2025Article
- Pathogenic mucorales: Deciphering their cell wall polysaccharidome and immunostimulatory potential.Virulence · 2025Article
- The β-1,6-glucanase MoGlu16 is Required for Mycelia Growth and Pathogenicity of Magnaporthe oryzae.Current microbiology · 2025Article
- Article
- Synergistic effect of synthetic derivatives of 1,3,4-thiadiazole with amphotericin B in antifungal therapy.Scientific reports · 2025Article
- β-1,6-Glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wall.eLife · 2024Article
- Spent Brewer's Yeast Lysis Enables aInternational journal of molecular sciences · 2024Review
- Comparative Analysis of the Aspergillus fumigatus Cell Wall Modification and Ensuing Human Dendritic Cell Responses by β-(1,3)-Glucan Synthase Inhibitors-Caspofungin and Enfumafungin.Mycopathologia · 2024Article
- Adaptative responses of Neurospora crassa by histidine kinases upon the attack of the arthropod Sinella curviseta.Current genetics · 2024Article
- Innovations in Antifungal Drug Discovery among Cell Envelope Synthesis Enzymes through Structural Insights.Journal of fungi (Basel, Switzerland) · 2024Review
- Article
- β-glucan: a potent adjuvant in immunotherapy for digestive tract tumors.Frontiers in immunology · 2024Review
- Functional analysis of a novel endo-β-1,6-glucanaseFrontiers in microbiology · 2024Article
- Yeast metagenomics: analytical challenges in the analysis of the eukaryotic microbiome.Microbiome research reports · 2024Review
- Solid-StateMolecules (Basel, Switzerland) · 2023Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite its essential role in the yeast cell wall, the exact composition of the beta-(1,6)-glucan component is not well characterized. While solubilizing the cell wall alkali-insoluble fraction from a wild type strain of Saccharomyces cerevisiae using a recombinant beta-(1,3)-glucanase followed by chromatographic characterization of the digest on an anion exchange column, we observed a soluble polymer that eluted at the end of the solvent gradient run. Further characterization indicated this soluble polymer to have a molecular mass of approximately 38 kDa and could be hydrolyzed only by beta-(1,6)-glucanase. Gas chromatography mass spectrometry and NMR ((1)H and (13)C) analyses confirmed it to be a beta-(1,6)-glucan polymer with, on average, branching at every fifth residue with one or two beta-(1,3)-linked glucose units in the side chain. This polymer peak was significantly reduced in the corresponding digests from mutants of the kre genes (kre9 and kre5) that are known to play a crucial role in the beta-(1,6)-glucan biosynthesis. In the current study, we have developed a biochemical assay wherein incubation of UDP-[(14)C]glucose with permeabilized S. cerevisiae yeasts resulted in the synthesis of a polymer chemically identical to the branched beta-(1,6)-glucan isolated from the cell wall. Using this assay, parameters essential for beta-(1,6)-glucan synthetic activity were defined.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.