ArticleCurrent biology : CB2009
The processivity of kinesin-2 motors suggests diminished front-head gating.
Article in Current biology : CB, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 65 citations in OpenAlex.
- How neurons maintain their axons long-term: an integrated view of axon biology and pathology.Frontiers in neuroscience · 2023Review
- Effect of detachment of motor protein from track on its transport.Journal of biological physics · 2022Article
- Functional exploration of heterotrimeric kinesin-II in IFT and ciliary length control ineLife · 2020Article
- The crowding dynamics of the motor protein kinesin-II.PloS one · 2020Article
- Article
- Directionally biased sidestepping of Kip3/kinesin-8 is regulated by ATP waiting time and motor-microtubule interaction strength.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Assessing the Impact of Electrostatic Drag on Processive Molecular Motor Transport.Bulletin of mathematical biology · 2018Article
- Kinesin-2 motors: Kinetics and biophysics.The Journal of biological chemistry · 2018Review
- Motor Reattachment Kinetics Play a Dominant Role in Multimotor-Driven Cargo Transport.Biophysical journal · 2018Article
- Intraflagellar transport: mechanisms of motor action, cooperation, and cargo delivery.The FEBS journal · 2017Review
- Intraflagellar transport velocity is governed by the number of active KIF17 and KIF3AB motors and their motility properties under load.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Kinesin Processivity Is Determined by a Kinetic Race from a Vulnerable One-Head-Bound State.Biophysical journal · 2017Article
- The axonal transport motor kinesin-2 navigates microtubule obstacles via protofilament switching.Traffic (Copenhagen, Denmark) · 2017Article
- The Kinesin-1 Chemomechanical Cycle: Stepping Toward a Consensus.Biophysical journal · 2016Review
- Kinetics of nucleotide-dependent structural transitions in the kinesin-1 hydrolysis cycle.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Kinesin Motor Enzymology: Chemistry, Structure, and Physics of Nanoscale Molecular Machines.Biophysical reviews · 2015Article
- Retrograde NGF axonal transport--motor coordination in the unidirectional motility regime.Biophysical journal · 2015Article
- The Mechanochemical Cycle of Mammalian Kinesin-2 KIF3A/B under Load.Current biology : CB · 2015Article
- Processivity of the kinesin-2 KIF3A results from rear head gating and not front head gating.The Journal of biological chemistry · 2015Article
- Transport by populations of fast and slow kinesins uncovers novel family-dependent motor characteristics important for in vivo function.Biophysical journal · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Kinesin-2 motors, which are involved in intraflagellar transport and cargo transport along cytoplasmic microtubules, differ from motors in the canonical kinesin-1 family by having a heterodimeric rather than homodimeric structure and possessing a three amino acid insertion in their neck linker domain. To determine how these structural features alter the chemomechanical coupling in kinesin-2, we used single-molecule bead experiments to measure the processivity and velocity of mouse kinesin-2 heterodimer (KIF3A/B) and the engineered homodimers KIF3A/A and KIF3B/B and compared their behavior to Drosophila kinesin-1 heavy chain (KHC). Single-motor run lengths of kinesin-2 were 4-fold shorter than those of kinesin-1. Extending the kinesin-1 neck linker by three amino acids led to a similar reduction in processivity. Furthermore, kinesin-2 processivity varied inversely with ATP concentration. Stochastic simulations of the kinesin-1 and kinesin-2 hydrolysis cycles suggest that "front-head gating," in which rearward tension prevents ATP binding to the front head when both heads are bound to the microtubule, is diminished in kinesin-2. Because the mechanical tension that underlies front-head gating must be transmitted through the neck linker domains, we propose that the diminished coordination in kinesin-2 is a result of its longer and, hence, more compliant neck linker element.
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