ArticleACS chemical biology2009
Small molecule microarrays of RNA-focused peptoids help identify inhibitors of a pathogenic group I intron.
Article in ACS chemical biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- Linker Optimization of cMYC RIBOTAC for Efficient cMYC Modulation.ChemMedChem · 2026Article
- The Druggable Transcriptome Project: From Chemical Probes to Precision Medicines.Biochemistry · 2025Review
- Group I introns: Structure, splicing and their applications in medical mycology.Genetics and molecular biology · 2024Article
- Targeting RNA structures with small molecules.Nature reviews. Drug discovery · 2022Review
- A Fluorescent Reverse-Transcription Assay to Detect Chemical Adducts on RNA.Biochemistry · 2022Article
- Article
- Selective Small Molecule Recognition of RNA Base Pairs.ACS combinatorial science · 2018Article
- Microarray-based technologies for the discovery of selective, RNA-binding molecules.Methods (San Diego, Calif.) · 2016Review
- Recent discoveries and applications involving small-molecule microarrays.Current opinion in chemical biology · 2014Review
- Identification of conserved RNA secondary structures at influenza B and C splice sites reveals similarities and differences between influenza A, B, and C.BMC research notes · 2014Article
- Probing a 2-aminobenzimidazole library for binding to RNA internal loops via two-dimensional combinatorial screening.ACS chemical biology · 2012Article
- Identifying the preferred RNA motifs and chemotypes that interact by probing millions of combinations.Nature communications · 2012Article
- The 3' splice site of influenza A segment 7 mRNA can exist in two conformations: a pseudoknot and a hairpin.PloS one · 2012Article
- Studying modification of aminoglycoside antibiotics by resistance-causing enzymes via microarray.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Extraordinarily robust polyproline type I peptoid helices generated via the incorporation of α-chiral aromatic N-1-naphthylethyl side chains.Journal of the American Chemical Society · 2011Article
- Evaluating target silencing by short hairpin RNA mediated by the group I intron in cultured mammalian cells.BMC biotechnology · 2011Article
- Biomolecularmodeling and simulation: a field coming of age.Quarterly reviews of biophysics · 2011Review
- Solid-phase synthesis of N-substituted glycine oligomers (alpha-peptoids) and derivatives.Molecules (Basel, Switzerland) · 2010Review
- The Privileged Chemical Space Predictor (PCSP): a computer program that identifies privileged chemical space from screens of modularly assembled chemical libraries.Bioorganic & medicinal chemistry letters · 2010Article
- Discovering ligands for a microRNA precursor with peptoid microarrays.Nucleic acids research · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Peptoids that inhibit the group I intron RNA from Candida albicans, an opportunistic pathogen that kills immunocompromised hosts, have been identified using microarrays. The arrayed peptoid library was constructed using submonomers with moieties similar to ones found in small molecules known to bind RNA. Library members that passed quality control analysis were spotted onto a microarray and screened for binding to the C. albicans group I intron ribozyme. Each ligand binder identified from microarray-based screening inhibited self-splicing in the presence of 1 mM nucleotide concentration of bulk yeast tRNA with IC(50)'s between 150 and 2200 microM. The binding signals and the corresponding IC(50)'s were used to identify features in the peptoids that predispose them for RNA binding. After statistical analysis of the peptoids' structures that bind, a second generation of inhibitors was constructed using these important features; all second generation inhibitors have improved potencies with IC(50)'s of <100 microM. The most potent inhibitor is composed of one phenylguanidine and three tryptamine submonomers and has an IC(50) of 31 microM. This compound is 6-fold more potent than pentamidine, a clinically used drug that inhibits self-splicing. These results show that (i) modulators of RNA function can be identified by designing RNA-focused chemical libraries and screening them via microarray; (ii) statistical analysis of ligand binders can identify features in leads that predispose them for binding to their targets; and (iii) features can then be programmed into second generation inhibitors to design ligands with improved potencies.
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