ArticleJournal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract2009
Esophagus tissue engineering: hybrid approach with esophageal epithelium and unidirectional smooth muscle tissue component generation in vitro.
Article in Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 66 citations in OpenAlex.
- Multi-layered Free-form 3D Cell-printed Tubular Construct with Decellularized Inner and Outer Esophageal Tissue-derived Bioinks.Scientific reports · 2020Article
- 3D Bioprinting Strategies for the Regeneration of Functional Tubular Tissues and Organs.Bioengineering (Basel, Switzerland) · 2020Review
- Comparison of esophageal submucosal glands in experimental models for esophagus tissue engineering applications.Esophagus : official journal of the Japan Esophageal Society · 2019Article
- Surgical perspectives regarding application of biomaterials for the management of large congenital diaphragmatic hernia defects.Pediatric surgery international · 2018Review
- The extracellular matrix of the gastrointestinal tract: a regenerative medicine platform.Nature reviews. Gastroenterology & hepatology · 2017Review
- Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation.Journal of visualized experiments : JoVE · 2017Article
- Murine and human tissue-engineered esophagus form from sufficient stem/progenitor cells and do not require microdesigned biomaterials.Tissue engineering. Part A · 2015Article
- Isolation of esophageal stem cells with potential for therapy.Pediatric surgery international · 2014Article
- Tissue engineering in the gut: developments in neuromusculature.Gastroenterology · 2014Review
- Tissue engineering and regenerative medicine as applied to the gastrointestinal tract.Current opinion in biotechnology · 2013Review
- In vitro development and characterization of a tissue-engineered conduit resembling esophageal wall using human and pig skeletal myoblast, oral epithelial cells, and biologic scaffolds.Tissue engineering. Part A · 2013Article
- Regenerative surgery: tissue engineering in general surgical practice.World journal of surgery · 2012Review
- Phenotypic changes in cultured smooth muscle cells: limitation or opportunity for tissue engineering of hollow organs?Journal of tissue engineering and regenerative medicine · 2012Article
- Intestinal tissue engineering: current concepts and future vision of regenerative medicine in the gut.Neurogastroenterology and motility · 2012Review
- Tissue engineering and regenerative medicine research perspectives for pediatric surgery.Pediatric surgery international · 2010Review
- Fluorescence-activated cell sorting of PCK-26 antigen-positive cells enables selection of ovine esophageal epithelial cells with improved viability on scaffolds for esophagus tissue engineering.Pediatric surgery international · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe aim of this study was to engineer the two main components of the esophagus in vitro: (a) esophageal epithelium and (b) smooth muscle tissue. Furthermore, (a) survivability of esophageal epithelial cells (EEC) on basement membrane matrix (BMM)-coated scaffolds and (b) oriented smooth muscle tissue formation on unidirectional BMM-coated collagen scaffolds was investigated.
methodsBoth EEC and smooth muscle cells (SMC) were sourced from Sprague-Dawley rats. The EEC were maintained in vitro and seeded onto BMM-coated 2-D collagen scaffolds. Similarly, smooth muscle cells were obtained using an explants technique and seeded on unidirectional 3-D BMM-coated collagen scaffolds. Cell-polymer constructs for EEC and SMC were maintained in vitro for 8 weeks.
resultsProtocols to obtain higher yield of EEC were established. EEC formed a layer of differentiated epithelium after 14 days. EEC survivability on polymers was observed up to 8 weeks. Unidirectional smooth muscle tissue strands were successfully engineered.
conclusionEsophageal epithelium generation, survivability of EEC on BMM-coated scaffolds, and engineering of unidirectional smooth muscle strands were successful in vitro. The hybrid approach of assembling individual tissue components in vitro using BMM-coated scaffolds and later amalgamating them to form composite tissue holds promises in the tissue engineering of complex organ systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.