ArticleMolecular genetics and genomics : MGG2009
Loss of mitochondrial DNA under genotoxic stress conditions in the absence of the yeast DNA helicase Pif1p occurs independently of the DNA helicase Rrm3p.
Article in Molecular genetics and genomics : MGG, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 19 citations in OpenAlex.
- The function of Mgs1/WRNIP1 in genome maintenance.Frontiers in molecular biosciences · 2026Review
- A system for inducible mitochondria-specific protein degradation in vivo.Nature communications · 2024Article
- Review
- Pif1 family DNA helicases: A helpmate to RNase H?DNA repair · 2019Review
- Structure and function of Pif1 helicase.Biochemical Society transactions · 2017Review
- PIF1 family DNA helicases suppress R-loop mediated genome instability at tRNA genes.Nature communications · 2017Article
- Analysis of Protein-protein Interaction Interface between Yeast Mitochondrial Proteins Rim1 and Pif1 Using Chemical Cross-linking Mass Spectrometry.Journal of proteomics & bioinformatics · 2015Article
- Relationship between gene duplicability and diversifiability in the topology of biochemical networks.BMC genomics · 2014Article
- Recombinogenic conditions influence partner choice in spontaneous mitotic recombination.PLoS genetics · 2013Article
- Physical and functional interaction between yeast Pif1 helicase and Rim1 single-stranded DNA binding protein.Nucleic acids research · 2013Article
- DNA replication through hard-to-replicate sites, including both highly transcribed RNA Pol II and Pol III genes, requires the S. pombe Pfh1 helicase.Genes & development · 2012Article
- The TPR-containing domain within Est1 homologs exhibits species-specific roles in telomerase interaction and telomere length homeostasis.BMC molecular biology · 2011Article
- Regulation of fragile sites expression in budding yeast by MEC1, RRM3 and hydroxyurea.Journal of cell science · 2011Article
- Unwinding the functions of the Pif1 family helicases.DNA repair · 2010Review
- A genome-wide screen in Saccharomyces cerevisiae reveals a critical role for the mitochondria in the toxicity of a trichothecene mycotoxin.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Mitochondrial helicases and mitochondrial genome maintenance.Mechanisms of ageing and developmentArticle
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How the cellular amount of mitochondrial DNA (mtDNA) is regulated under normal conditions and in the presence of genotoxic stress is less understood. We demonstrate that the inefficient mtDNA replication process of mutant yeast cells lacking the PIF1 DNA helicase is partly rescued in the absence of the DNA helicase RRM3. The rescue effect is likely due to the increase in the deoxynucleoside triphosphates (dNTPs) pool caused by the lack of RRM3. In contrast, the Pif1p-dependent mtDNA breakage in the presence and absence of genotoxic stress is not suppressed if RRM3 is lacking suggesting that this phenotype is likely independent of the dNTP pool. Pif1 protein (Pif1p) was found to stimulate the incorporation of dNTPs into newly synthesised mtDNA of gradient-purified mitochondria. We propose that Pif1p that acts likely as a DNA helicase in mitochondria affects mtDNA replication directly. Possible roles of Pif1p include the resolution of secondary DNA and/or DNA/RNA structures, the temporarily displacement of tightly bound mtDNA-binding proteins, or the stabilization of the mitochondrial replication complex during mtDNA replication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.