ArticleMolecular therapy : the journal of the American Society of Gene Therapy2009
Baculovirus transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 53 citations in OpenAlex.
- Can pluripotent/multipotent stem cells reverse Parkinson's disease progression?Frontiers in neuroscience · 2024Review
- Solutions against emerging infectious and noninfectious human diseases through the application of baculovirus technologies.Applied microbiology and biotechnology · 2021Review
- Coactivation of Endogenous Wnt10b and Foxc2 by CRISPR Activation Enhances BMSC Osteogenesis and Promotes Calvarial Bone Regeneration.Molecular therapy : the journal of the American Society of Gene Therapy · 2020Article
- Beneficial Effect of TaON-Ag Nanocomposite Titanium on Antibacterial Capacity in Orthopedic Application.International journal of nanomedicine · 2020Article
- BMP-2 Gene Delivery-Based Bone Regeneration in Dentistry.Pharmaceutics · 2019Review
- Global Screening of Antiviral Genes that Suppress Baculovirus Transgene Expression in Mammalian Cells.Molecular therapy. Methods & clinical development · 2017Article
- Adenoviral vector encoding soluble Flt-1 engineered human endometrial mesenchymal stem cells effectively regress endometriotic lesions in NOD/SCID mice.Gene therapy · 2016Article
- Polymeric Nanohybrids as a New Class of Therapeutic Biotransporters.Macromolecular chemistry and physics · 2016Article
- Genetic rearrangements of variable di-residue (RVD)-containing repeat arrays in a baculoviral TALEN system.Molecular therapy. Methods & clinical development · 2014Article
- Regenerating cartilages by engineered ASCs: prolonged TGF-β3/BMP-6 expression improved articular cartilage formation and restored zonal structure.Molecular therapy : the journal of the American Society of Gene Therapy · 2014Article
- Adaptive immune responses elicited by baculovirus and impacts on subsequent transgene expression in vivo.Journal of virology · 2013Article
- Development of the hybrid Sleeping Beauty: baculovirus vector for sustained gene expression and cancer therapy.Gene therapy · 2012Article
- Defective antiviral responses of induced pluripotent stem cells to baculoviral vector transduction.Journal of virology · 2012Article
- Targeted suicide gene therapy for glioma using human embryonic stem cell-derived neural stem cells genetically modified by baculoviral vectors.Gene therapy · 2012Article
- Angiopoietin-1-expressing adipose stem cells genetically modified with baculovirus nanocomplex: investigation in rat heart with acute infarction.International journal of nanomedicine · 2012Article
- Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.PloS one · 2012Article
- Baculovirus-transduced bone marrow mesenchymal stem cells for systemic cancer therapy.Cancer gene therapy · 2010Article
- Recombinant baculovirus as a highly potent vector for gene therapy of human colorectal carcinoma: molecular cloning, expression, and in vitro characterization.Molecular biotechnology · 2010Article
- Baculovirus transduction of mesenchymal stem cells triggers the toll-like receptor 3 pathway.Journal of virology · 2009Article
- Baculovirus as a gene delivery vector: recent understandings of molecular alterations in transduced cells and latest applications.Biotechnology advancesReview
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Baculovirus holds great promise for the genetic modification of mesenchymal stem cells (MSCs). However, whether baculovirus transduction provokes undesired MSCs responses that might compromise their in vivo applications has yet to be examined. Hereby, we unraveled that baculovirus transduction of human MSCs upregulated the transcription of interleukin (IL)-1beta, interferon (IFN)-alpha and IL-6, but not tumor necrosis factor (TNF)-alpha and IFN-gamma. However, only IL-6 secretion was detectable by enzyme-linked immunosorbent assay (ELISA). Baculovirus transduction also stimulated transient, low level upregulation of human leukocyte antigen I (HLA-I) on the human MSCs surface, yet it did not either altered the HLA-II expression or impaired the MSCs ability to inhibit lymphocyte proliferation. After transplantation into allogeneic rats, the transduced rat MSCs elicited transient, mild macrophage responses, but the cells remained tolerant as judged by the persistence of transplanted cells and absence of CD8(+) T cells infiltration. Besides, transplantation of the transduced MSCs did not provoke systemic induction of monocytes and CD8(+) T cells. This study, for the first time, explores the responses of MSCs to virus transduction and confirms the safety of transplanting baculovirus-engineered MSCs into immunocompetent animals for cell-based gene therapy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.