ArticleAmerican journal of physiology. Endocrinology and metabolism2009
Role for cumulus cell-produced EGF-like ligands during primate oocyte maturation in vitro.
Article in American journal of physiology. Endocrinology and metabolism, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Effects of dietary palygorskite supplementation on production performance and reproductive function of laying hens.Poultry science · 2026Article
- Betacellulin regulates gap junction intercellular communication by inducing the phosphorylation of connexin 43 in human granulosa-lutein cells.Journal of ovarian research · 2023Article
- The role of amphiregulin in ovarian function and disease.Cellular and molecular life sciences : CMLS · 2023Review
- Treatment of Epidermal Growth Factor (EGF) enhances Nuclear Maturation of Porcine Oocytes and Stimulates Expression of ER/Golgi Transport Proteins.Development & reproduction · 2017Article
- Matrix metalloproteinase 2 level in human follicular fluid is a reliable marker of human oocyte maturation in in vitro fertilization and intracytoplasmic sperm injection cycles.Reproductive biology and endocrinology : RB&E · 2015Article
- The potential role of granulosa cells in the maturation rate of immature human oocytes and embryo development: A co-culture study.Clinical and experimental reproductive medicine · 2015Article
- Disruptions in follicle cell functions in the ovaries of rhesus monkeys during summer.Physiological genomics · 2015Article
- EGF-like ligands mediate progesterone's anti-apoptotic action on macaque granulosa cells.Biology of reproduction · 2013Article
- Rhesus monkey cumulus cells revert to a mural granulosa cell state after an ovulatory stimulus.Endocrinology · 2012Article
- Regulation of oocyte mitochondrial DNA copy number by follicular fluid, EGF, and neuregulin 1 during in vitro maturation affects embryo development in pigs.Theriogenology · 2012Article
- Amphiregulin promotes the maturation of oocytes isolated from the small antral follicles of the rhesus macaque.Human reproduction (Oxford, England) · 2012Article
- The effect of human cumulus cells on the maturation and developmental potential of immature oocytes in ICSI cycles.Journal of assisted reproduction and genetics · 2012Article
- Extensive effects of in vitro oocyte maturation on rhesus monkey cumulus cell transcriptome.American journal of physiology. Endocrinology and metabolism · 2011Article
- Human oocyte maturation is dependent on LH-stimulated accumulation of the epidermal growth factor-like growth factor, amphiregulin.Human reproduction (Oxford, England) · 2010Article
- The nuclear receptor cofactor receptor-interacting protein 140 is a positive regulator of amphiregulin expression and cumulus cell-oocyte complex expansion in the mouse ovary.Endocrinology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The developmental competence of in vitro-matured (IVM) rhesus macaque cumulus oocyte complexes (COCs) is deficient compared with in vivo-matured (IVM) oocytes. To improve oocyte quality and subsequent embryo development following IVM, culture conditions must be optimized. A series of experiments was undertaken to determine the role of epidermal growth factor (EGF) during IVM of rhesus macaque COCs. The addition of Tyrphostin AG-1478 (a selective inhibitor of the EGF receptor EGFR) to the IVM medium yielded fewer oocytes maturing to metaphase II of meiosis II (MII), decreased cumulus expansion, and a lower percentage of embryos that developed to the blastocyst stage compared with untreated IVM controls, indicating that EGFR activation is important for IVM maturation in the rhesus macaque. However, the addition of recombinant human EGF (r-hEGF) to the IVM medium did not enhance outcome. The expression of mRNAs encoding the EGF-like factors amphiregulin, epiregulin, and betacellulin in cumulus cells indicates that these factors produced by cumulus cells may be responsible for maximal EGFR activation during oocyte maturation, precluding any further effect of exogenous r-hEGF. Additionally, these results illustrate the potential futility of exogenous supplementation of IVM medium without prior knowledge of pathway activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.