ArticleThe Journal of clinical endocrinology and metabolism2009
Progestin and thrombin regulate tissue factor expression in human term decidual cells.
Article in The Journal of clinical endocrinology and metabolism, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 3 syntheses or guidelines pooled it, 54 citations in OpenAlex.
- Molecular Changes on Maternal-Fetal Interface in Placental Abruption-A Systematic Review.International journal of molecular sciences · 2021Pooled it
- Association between progestin-only contraceptive use and cardiometabolic outcomes: A systematic review and meta-analysis.European journal of preventive cardiology · 2018Pooled it
- Assessing the risk of venous thromboembolic events in women taking progestin-only contraception: a meta-analysis.BMJ (Clinical research ed.) · 2012Pooled it
- Enhanced ZBTB16 Levels by Progestin-Only Contraceptives Induces Decidualization and Inflammation.International journal of molecular sciences · 2023Article
- Spontaneous preterm birth: Involvement of multiple feto-maternal tissues and organ systems, differing mechanisms, and pathways.Frontiers in endocrinology · 2022Review
- Regulation of Proinflammatory Molecules and Tissue Factor by SARS-CoV-2 Spike Protein in Human Placental Cells: Implications for SARS-CoV-2 Pathogenesis in Pregnant Women.Frontiers in immunology · 2022Article
- Single-cell transcriptomics analysis showing functional heterogeneity in decidual stromal cells during labor.Journal of investigative medicine : the official publication of the American Federation for Clinical Research · 2020Article
- The Use of Tranexamic Acid to Prevent Postpartum Hemorrhage.Journal of midwifery & women's health · 2020Article
- Thrombin-Induced Decidual Colony-Stimulating Factor-2 Promotes Abruption-Related Preterm Birth by Weakening Fetal Membranes.The American journal of pathology · 2020Article
- Toll-like receptor 9, maternal cell-free DNA and myometrial cell response to CpG oligodeoxynucleotide stimulation.American journal of reproductive immunology (New York, N.Y. : 1989) · 2019Article
- Expression of flTF and asTF splice variants in various cell strains and tissues.Molecular medicine reports · 2019Article
- Decreased LIN28B in preeclampsia impairs human trophoblast differentiation and migration.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- D-Dimer in African Americans: Whole Genome Sequence Analysis and Relationship to Cardiovascular Disease Risk in the Jackson Heart Study.Arteriosclerosis, thrombosis, and vascular biology · 2017Observational
- The role of decidual cells in uterine hemostasis, menstruation, inflammation, adverse pregnancy outcomes and abnormal uterine bleeding.Human reproduction update · 2016Review
- Agonist-Dependent Downregulation of Progesterone Receptors in Human Cervical Stromal Fibroblasts.Reproductive sciences (Thousand Oaks, Calif.) · 2016Article
- Expression of Interferon γ by Decidual Cells and Natural Killer Cells at the Human Implantation Site: Implications for Preeclampsia, Spontaneous Abortion, and Intrauterine Growth Restriction.Reproductive sciences (Thousand Oaks, Calif.) · 2015Article
- Abruption-induced preterm delivery is associated with thrombin-mediated functional progesterone withdrawal in decidual cells.The American journal of pathology · 2012Article
- Toll-like receptor 4 expression in decidual cells and interstitial trophoblasts across human pregnancy.American journal of reproductive immunology (New York, N.Y. : 1989) · 2012Article
- miR-93/106b and their host gene, MCM7, are differentially expressed in leiomyomas and functionally target F3 and IL-8.Molecular endocrinology (Baltimore, Md.) · 2012Article
- Preeclampsia, hypoxia, thrombosis, and inflammation.Journal of pregnancy · 2012Review
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
contextPerivascular cell membrane-bound tissue factor (TF) initiates hemostasis via thrombin generation. The identity and potential regulation of TF-expressing cells at the human maternal-fetal interface that confers hemostatic protection during normal and preterm delivery is unclear.
objectivesThe objective of the study were to identify TF-expressing cells at the maternal-fetal interface in term and preterm decidual sections by immunohistochemistry and evaluate progestin, thrombin, TNF-alpha, and IL-1beta effects on TF expression by cultured human term decidual cells (DCs). INTERVENTIONS AND
main outcome measuresSerial placental sections were immunostained for TF. Leukocyte-free term DC monolayers were incubated with 10(-8) M estradiol (E2) or E2 plus 10(-7) M medroxyprogestrone acetate (MPA) +/- thrombin or TNF-alpha or IL-1beta. ELISA and Western blotting assessed TF in cell lysates. Quantitative real-time RT-PCR measured TF mRNA levels.
resultsImmunolocalized TF in DC membranes in preterm and term placental sections displayed higher Histologic Scores than villous mesenchymal cells (P < 0.05). TF was undetected in interstitial or extravillous trophoblasts. Compared with DCs incubated with E2, MPA and 2.5 U/ml thrombin each doubled TF levels (P < 0.05) and E2 + MPA + thrombin further doubled TF levels (P < 0.05), whereas TNF-alpha and IL-1beta were ineffective. Western blotting confirmed the ELISA results. Quantitative RT-PCR revealed corresponding changes in TF mRNA levels.
conclusionsIn human term placental sections, DC-expressed TF exceeds that of other cell types at the maternal-fetal interface and is localized at the cell membranes in which it can bind to factor VII and meet the hemostatic demands of labor and delivery via thrombin formation. Unlike the general concept that TF is constitutive in cells that highly express it, MPA and thrombin significantly enhanced TF expression in term DC monolayers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.