Evidence map›Paper›PMID 19276076›Full record

ArticleThe Journal of biological chemistry2009

Cyclin D3 mediates synthesis of a hyaluronan matrix that is adhesive for monocytes in mesangial cells stimulated to divide in hyperglycemic medium.

Juan Ren, Vincent C Hascall, Aimin Wang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
3.2field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 40 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. The journey of hyaluronan research in theThe Journal of biological chemistry · 2019
    Review
  7. Biological roles of glycans.Glycobiology · 2017
    Review
  8. Molecular Functions of Glycoconjugates in Autophagy.Journal of molecular biology · 2016
    Review
  9. Article
  10. Review
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Chain gangs: new aspects of hyaluronan metabolism.Biochemistry research international · 2012
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Juan RenFrom the Department of Biomedical Engineering, Cleveland Clinic, Cleveland, Ohio 44195.
Vincent C HascallFrom the Department of Biomedical Engineering, Cleveland Clinic, Cleveland, Ohio 44195.
Aimin WangFrom the Department of Biomedical Engineering, Cleveland Clinic, Cleveland, Ohio 44195. Electronic address: wanga@ccf.org.
Cleveland Clinic · US

Funding

Robotics CoreP30AR050953 · NIAMS · CLEVELAND CLINIC LERNER COM-CWRU · PI HASCALL, VINCENT CHARLES · 2004 to 2008
$2.9M
Monocyte Adhesive Hyaluronan Structures in DiabetesR01DK062934 · NIDDK · CLEVELAND CLINIC LERNER COM-CWRU · PI WANG, AIMIN · 2004 to 2008
$1.2M
NIAMS NIH HHS 1P30 AR-050953NIAMS NIH HHS P30 AR050953NIDDK NIH HHS R01 DK062934NIDDK NIH HHS R01 DK62934
6 · The paper itself

Abstract

Serum-starved, growth-arrested, near confluent rat mesangial cell cultures were stimulated to divide in medium with low (5.6 mM) or high (25.6 mM) glucose. In high glucose cultures Western blots showed large increases in cyclin D3 and CCAAT/enhancer-binding protein alpha (C/EBPalpha) at 48-72 h, concurrent with the production of a monocyte-adhesive hyaluronan matrix, whereas low glucose and mannitol osmotic control cultures did not. Cyclin D3 small interfering RNA inhibited both the synthesis of this matrix and the up-regulation of C/EBPalpha in cultures exposed to high glucose, indicating that cyclin D3 is a key mediator in regulating responses of dividing mesangial cells to hyperglycemia. A complex with cyclin D3, cyclin-dependent kinase 4, and C/EBPalpha was observed at 48-72 h in the hyperglycemic cultures, and cyclin D3 and C/EBPalpha were spatially co-localized in coalesced perinuclear honeycomb-like structures with embedded hyaluronan. Furthermore, microtubule-associated protein 1 light chain 3, a marker for autophagy, colocalizes with these structures. These results suggest that cyclin D3 is a central coordinator that controls the organization of a complex set of proteins that regulate autophagy, formation of the monocyte-adhesive hyaluronan matrix, and C/EBPalpha-mediated lipogenesis. Abnormal deposits of hyaluronan, cyclin D3, and C/EBPalpha were present in glomeruli of kidney sections from hyperglycemic rats 4 weeks after streptozotocin treatment, indicating that similar processes likely occur in vivo. Mesangial cell cultures treated with poly(I:C) or tunicamycin in normal glucose media synthesized monocyte-adhesive hyaluronan matrices but with concurrent down-regulation of cyclin D3. This indicates that the cyclin D3 mechanism is induced by hyperglycemia and is distinct from those involved in these cell stress responses.

Indexed as

AnimalsAnti-Bacterial AgentsAutophagyCCAAT-Enhancer-Binding Protein-alphaCell AdhesionCell DivisionCells, CulturedCulture Media, Serum-FreeCyclin D3CyclinsExtracellular MatrixGlucoseHyaluronic AcidHyperglycemiaMaleMesangial CellsAnti-Bacterial AgentsCCAAT-Enhancer-Binding Protein-alphaCcnd3 protein, ratCulture Media, Serum-FreeCyclin D3CyclinsGlucoseHyaluronic AcidTunicamycin

Identifiers

PMID19276076
PMCPMC2713523
OpenAlexW2001113268

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.