ArticleBiochemical and biophysical research communications2009
NMR study of the cataract-linked P23T mutant of human gammaD-crystallin shows minor changes in hydrophobic patches that reflect its retrograde solubility.
Article in Biochemical and biophysical research communications, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 29 citations in OpenAlex.
- A missense mutation in CRYGD linked with autosomal dominant congenital cataract of aculeiform type.Molecular and cellular biochemistry · 2012Trial
- Modeling congenital cataract in vitro using patient-specific induced pluripotent stem cells.NPJ Regenerative medicine · 2021Article
- Human αB-crystallin discriminates between aggregation-prone and function-preserving variants of a client protein.Biochimica et biophysica acta. General subjects · 2020Article
- Model for screened, charge-regulated electrostatics of an eye lens protein: Bovine gammaB-crystallin.Physical review. E · 2017Article
- Nuclear Magnetic Resonance Structure of a Major Lens Protein, Human γC-Crystallin: Role of the Dipole Moment in Protein Solubility.Biochemistry · 2016Article
- Stability of Protein-Specific Hydration Shell on Crowding.Journal of the American Chemical Society · 2016Article
- Molecular mechanism of the chaperone function of mini-α-crystallin, a 19-residue peptide of human α-crystallin.Biochemistry · 2015Article
- Article
- Structural integrity of the Greek key motif in βγ-crystallins is vital for central eye lens transparency.PloS one · 2013Article
- Patterns of gene expression in microarrays and expressed sequence tags from normal and cataractous lenses.Human genomics · 2012Article
- Structural and biochemical characterization of the childhood cataract-associated R76S mutant of human γD-crystallin.Biochemistry · 2012Article
- Increased hydrophobicity and decreased backbone flexibility explain the lower solubility of a cataract-linked mutant of γD-crystallin.Journal of molecular biology · 2011Article
- Blind attraction: the mechanism of an inherited congenital cataract.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Altered chaperone-like activity of alpha-crystallins promotes cataractogenesis.The Journal of biological chemistry · 2010Article
- Structural and aggregation behavior of the human γD-crystallin mutant E107A, associated with congenital nuclear cataract.Molecular vision · 2010Article
- Increase in surface hydrophobicity of the cataract-associated P23T mutant of human gammaD-crystallin is responsible for its dramatically lower, retrograde solubility.Biochemistry · 2010Article
- Founder heterozygous P23T CRYGD mutation associated with cerulean (and coralliform) cataract in 2 Saudi families.Molecular vision · 2009Article
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6 authors at 2 institutions in 1 country.
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Abstract
The Pro23 to Thr (P23T) mutation in human gammaD-crystallin (HGD) shows several cataract phenotypes. We found earlier [A. Pande, O. Annunziata, N. Asherie, O. Ogun, G.B. Benedek, J. Pande, Decrease in protein solubility and cataract formation caused by the Pro23 to Thr mutation in human gamma D-crystallin, Biochemistry 44 (2005) 2491-2500] that the mutation dramatically lowers the solubility of P23T but the overall protein fold is maintained. Recently we observed that solutions of P23T showed liquid-liquid phase transition behavior similar to that of HGD but the liquid-protein crystal phase transition was altered, suggesting an asymmetric distribution of "sticky" patches on the protein surface [J.J. McManus, A. Lomakin, O. Ogun, A. Pande, M. Basan, J. Pande, G.B. Benedek, Altered phase diagram due to a single point mutation in human gammaD-crystallin, Proc. Natl. Acad. Sci. USA 104 (2007) 16856-16861]. Here we present high-resolution NMR studies of HGD and P23T in which we have made nearly complete backbone assignments. The data provide a structural basis for explaining the retrograde solubility of P23T by (a) identifying possible "sticky" patches on the surface of P23T and (b) highlighting their asymmetric distribution.
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