ArticlePloS one2009
Characterisation of early mucosal and neuronal lesions following Shigella flexneri infection in human colon.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
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- Serine Protease Autotransporters of theMicroorganisms · 2019Review
- Human Intestinal Enteroids as a Model System ofInfection and immunity · 2019Article
- Characterization of Diarrheagenic EnteroaggregativeFrontiers in cellular and infection microbiology · 2018Article
- Shigella depends on SepA to destabilize the intestinal epithelial integrity via cofilin activation.Gut microbes · 2017Article
- How Do the Virulence Factors ofFrontiers in cellular and infection microbiology · 2017Review
- Sirtuin-3 Is Expressed by Enteric Neurons but It Does not Play a Major Role in Their Regulation of Oxidative Stress.Frontiers in cellular neuroscience · 2016Article
- Adeno-Associated Viral Vector Delivery to the Enteric Nervous System: A Review.Postdoc journal : a journal of postdoctoral research and postdoctoral affairs · 2015Article
- Bioimage analysis of Shigella infection reveals targeting of colonic crypts.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- B lymphocytes undergo TLR2-dependent apoptosis upon Shigella infection.The Journal of experimental medicine · 2014Article
- Bacterial serine proteases secreted by the autotransporter pathway: classification, specificity, and role in virulence.Cellular and molecular life sciences : CMLS · 2014Review
- Initiation of an inflammatory response in resident intestinal lamina propria cells -use of a human organ culture model.PloS one · 2014Article
- Article
- Pathogenesis of human enterovirulent bacteria: lessons from cultured, fully differentiated human colon cancer cell lines.Microbiology and molecular biology reviews : MMBR · 2013Review
- Full-field optical coherence microscopy is a novel technique for imaging enteric ganglia in the gastrointestinal tract.Neurogastroenterology and motility · 2012Article
- Preventing acute gut wall damage in infectious diarrhoeas with glycosylated dendrimers.EMBO molecular medicine · 2012Article
- Genomic characterization of enteroaggregative Escherichia coli from children in Mali.The Journal of infectious diseases · 2012Article
- Shigellosis update: advancing antibiotic resistance, investment empowered vaccine development, and green bananas.Current opinion in infectious diseases · 2010Review
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundShigella, an enteroinvasive bacteria induces a major inflammatory response responsible for acute rectocolitis in humans. However, early effect of Shigella flexneri (S. flexneri) infection upon the human mucosa and its microenvironement, in particular the enteric nervous system, remains currently unknown. Therefore, in this study, we sought to characterize ex vivo the early events of shigellosis in a model of human colonic explants. In particular, we aimed at identifying factors produced by S. flexneri and responsible for the lesions of the barrier. We also aimed at determining the putative lesions of the enteric nervous system induced by S. flexneri. METHODOLOGY/PRINCIPAL
findingsWe first showed that, following 3 h of infection, the invasive but not the non-invasive strain of S. flexneri induced significant desquamation of the intestinal epithelial barrier and a reduction of epithelial height. These changes were significantly reduced following infection with SepA deficient S. flexneri strains. Secondly, S. flexneri induced rapid neuronal morphological alterations suggestive of cell death in enteric submucosal neurones. These alterations were associated with a significant increase in the proportion of vasoactive intestinal peptide (VIP) immunoreactive (IR) neurons but not in total VIP levels. The NMDA receptor antagonist MK-801 blocked neuronal morphological changes induced by S. flexneri, but not the increase in the proportion of VIP-IR. CONCLUSIONS/SIGNIFICANCE: This human explant model can be used to gain better insight into the early pathogenic events following S. flexneri infection and the mechanisms involved.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.