ArticleThe Journal of cell biology2009
ER membrane-bending proteins are necessary for de novo nuclear pore formation.
Article in The Journal of cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
95 citing papers in PubMed, 1 synthesis or guideline pooled it, 138 citations in OpenAlex.
- Transcriptomic analysis of castration, chemo-resistant and metastatic prostate cancer elucidates complex genetic crosstalk leading to disease progression.Functional & integrative genomics · 2021Pooled it
- Mitochondrial Dysfunction and Endoplasmic Reticulum Stress in Chronic Pain.Brain sciences · 2026Review
- PNET1 is a key regulator of NPC dynamics and cell division.Nature plants · 2025Article
- Baseline unfolded protein response signaling adjusts the timing of the mammalian cell cycle.Molecular biology of the cell · 2024Article
- Hva22, a REEP family protein in fission yeast, promotes reticulophagy in collaboration with a receptor protein.Autophagy · 2023Article
- SMPD4 regulates mitotic nuclear envelope dynamics and its loss causes microcephaly and diabetes.Brain : a journal of neurology · 2023Article
- Uip4p modulates nuclear pore complex function inNucleus (Austin, Tex.) · 2022Article
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- Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation.The Journal of cell biology · 2022Article
- Review
- The LEM-ESCRT toolkit: Repair and maintenance of the nucleus.Frontiers in cell and developmental biology · 2022Review
- Spatiotemporal Dynamic Regulation of Organelles During Meiotic Development, Insights From Fungi.Frontiers in cell and developmental biology · 2022Article
- Unexplored Cdc42 functions at the budding yeast nucleus suggested by subcellular localization.Small GTPases · 2022Article
- Review
- A distinct inner nuclear membrane proteome in Saccharomyces cerevisiae gametes.G3 (Bethesda, Md.) · 2021Article
- Article
- Regulation of ER Composition and Extent, and Putative Action in Protein Networks by ER/NE Protein TMEM147.International journal of molecular sciences · 2021Article
- Analysis of the Putative Nucleoporin POM33 in the Filamentous FungusJournal of fungi (Basel, Switzerland) · 2021Article
- Mechanism of membrane-curvature generation by ER-tubule shaping proteins.Nature communications · 2021Article
35 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Nucleocytoplasmic transport occurs exclusively through nuclear pore complexes (NPCs) embedded in pores formed by inner and outer nuclear membrane fusion. The mechanism for de novo pore and NPC biogenesis remains unclear. Reticulons (RTNs) and Yop1/DP1 are conserved membrane protein families required to form and maintain the tubular endoplasmic reticulum (ER) and the postmitotic nuclear envelope. In this study, we report that members of the RTN and Yop1/DP1 families are required for nuclear pore formation. Analysis of Saccharomyces cerevisiae prp20-G282S and nup133 Delta NPC assembly mutants revealed perturbations in Rtn1-green fluorescent protein (GFP) and Yop1-GFP ER distribution and colocalization to NPC clusters. Combined deletion of RTN1 and YOP1 resulted in NPC clustering, nuclear import defects, and synthetic lethality with the additional absence of Pom34, Pom152, and Nup84 subcomplex members. We tested for a direct role in NPC biogenesis using Xenopus laevis in vitro assays and found that anti-Rtn4a antibodies specifically inhibited de novo nuclear pore formation. We hypothesize that these ER membrane-bending proteins mediate early NPC assembly steps.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.