ArticleThe Journal of physiology2009
Single molecule measurements of mechanical interactions within ternary SNARE complexes and dynamics of their disassembly: SNAP25 vs. SNAP23.
Article in The Journal of physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
- Excitotoxic Storms of Ischemic Stroke: A Non-neuronal Perspective.Molecular neurobiology · 2024Review
- Pre-and Postfusion Tuning of Regulated Exocytosis by Cell Metabolites.Function (Oxford, England) · 2023Article
- Probing single molecule mechanical interactions of syntaxin 1A with native synaptobrevin 2 residing on a secretory vesicle.Cell calcium · 2022Article
- Ability of human SNAP-23 to generate high molecular weight SDS-resistant ternary SNARE complexes is influenced by C-terminal coil content.Biochemistry and biophysics reports · 2021Article
- Preface for the Vladimir Parpura Honorary Issue of Neurochemical Research.Neurochemical research · 2021Article
- Exocytotic fusion pore under stress.Cell stress · 2020Review
- Article
- Gliocrine System: Astroglia as Secretory Cells of the CNS.Advances in experimental medicine and biology · 2019Review
- Improving estimation of kinetic parameters in dynamic force spectroscopy using cluster analysis.The Journal of chemical physics · 2018Article
- Enteric glia regulate gut motility in health and disease.Brain research bulletin · 2018Review
- Exocytotic pore in a SNARE.Oncotarget · 2017Article
- Astroglial Vesicular Trafficking in Neurodegenerative Diseases.Neurochemical research · 2017Article
- Opposing roles for SNAP23 in secretion in exocrine and endocrine pancreatic cells.The Journal of cell biology · 2016Article
- Review
- Astrocytes as secretory cells of the central nervous system: idiosyncrasies of vesicular secretion.The EMBO journal · 2016Review
- Excitable Astrocytes: Ca(2+)- and cAMP-Regulated Exocytosis.Neurochemical research · 2015Review
- Nanopore sensing of botulinum toxin type B by discriminating an enzymatically cleaved Peptide from a synaptic protein synaptobrevin 2 derivative.ACS applied materials & interfaces · 2015Article
- Quantitative analysis linking inner hair cell voltage changes and postsynaptic conductance change: a modelling study.BioMed research international · 2015Article
- Pathological role for exocytotic glutamate release from astrocytes in hepatic encephalopathy.Current neuropharmacology · 2014Article
- Single-molecule measurements of dissociation rates and energy landscapes of binary trans snare complexes in parallel versus antiparallel orientation.Biophysical journal · 2011Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Regulated exocytosis is a crucial event for intercellular communication between neurons and astrocytes within the CNS. The soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) complex, composed of synaptobrevin 2, syntaxin and synaptosome-associated protein of 25 kDa or 23 kDa (SNAP25 or SNAP23), is essential in this process. It was reported that SNAP25 and SNAP23 have distinct roles in exocytotic release, where SNAP25, but not SNAP23, supports an exocytotic burst. It is not clear, however, whether this is due to the intrinsic properties of the ternary SNARE complex, containing either SNAP25 or SNAP23, or perhaps due to the differential association of these proteins with ancillary proteins to the complex. Here, using force spectroscopy, we show from single molecule investigations of the SNARE complex, that SNAP23A created a local interaction at the ionic layer by cuffing syntaxin 1A and synaptobrevin 2, similar to the action of SNAP25B; thus either of the ternary complexes would allow positioning of vesicles at a maximal distance of approximately 13 nm from the plasma membrane. However, the stability of the ternary SNARE complex containing SNAP23A is less than half of that for the complex containing SNAP25B. Thus, differences in the stability of the two different ternary complexes could underlie some of the SNAP25/23 differential ability to control the exocytotic burst.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.