ArticleInfection and immunity2009
Campylobacter jejuni cocultured with epithelial cells reduces surface capsular polysaccharide expression.
Article in Infection and immunity, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
- Temporal dynamics of gene expression during the development ofMicrobial genomics · 2025Article
- Pathogenicity and virulence ofVirulence · 2024Review
- Consortium ofAntibiotics (Basel, Switzerland) · 2024Article
- Review
- Whole-Genome and Plasmid Comparative Analysis of Campylobacter jejuni from Human Patients in Toyama, Japan, from 2015 to 2019.Microbiology spectrum · 2023Article
- Mixtures of natural antimicrobials can reduce Campylobacter jejuni, Salmonella enterica and Clostridium perfringens infections and cellular inflammatory response in MDCK cells.Gut pathogens · 2021Article
- Campylobacter sp.: Pathogenicity factors and prevention methods-new molecular targets for innovative antivirulence drugs?Applied microbiology and biotechnology · 2020Review
- Virulence Traits of InpatientMicroorganisms · 2020Article
- RevisitingFrontiers in cellular and infection microbiology · 2020Review
- Virulence of a T6SS Campylobacter jejuni chicken isolate from North Romania.BMC research notes · 2019Article
- TheFrontiers in microbiology · 2019Article
- Comparison of genomes and proteomes of four whole genome-sequenced Campylobacter jejuni from different phylogenetic backgrounds.PloS one · 2018Article
- A Novel Natural Antimicrobial Can Reduce theFrontiers in microbiology · 2018Article
- Virulence characteristics of hcp (+) Campylobacter jejuni and Campylobacter coli isolates from retail chicken.Gut pathogens · 2015Article
- Phenotypes of Campylobacter jejuni luxS mutants are depending on strain background, kind of mutation and experimental conditions.PloS one · 2014Article
- Virulence characteristics of five new Campylobacter jejuni chicken isolates.Gut pathogens · 2013Article
- Enhanced, sialoadhesin-dependent uptake of Guillain-Barre syndrome-associated Campylobacter jejuni strains by human macrophages.Infection and immunity · 2013Article
- Identification of a functional type VI secretion system in Campylobacter jejuni conferring capsule polysaccharide sensitive cytotoxicity.PLoS pathogens · 2013Article
- Cj1411c encodes for a cytochrome P450 involved in Campylobacter jejuni 81-176 pathogenicity.PloS one · 2013Article
- Mucosal reactive oxygen species decrease virulence by disrupting Campylobacter jejuni phosphotyrosine signaling.Cell host & microbe · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The host cell environment can alter bacterial pathogenicity. We employed a combination of cellular and molecular techniques to study the expression of Campylobacter jejuni polysaccharides cocultured with HCT-8 epithelial cells. After two passages, the amount of membrane-bound high-molecular-weight polysaccharide was considerably reduced. Microarray profiling confirmed significant downregulation of capsular polysaccharide (CPS) locus genes. Experiments using conditioned media showed that sugar depletion occurred only when the bacterial and epithelial cells were cocultured. CPS depletion occurred when C. jejuni organisms were exposed to conditioned media from a different C. jejuni strain but not when exposed to conditioned media from other bacterial species. Proteinase K or heat treatment of conditioned media under coculture conditions abrogated the effect on the sugars, as did formaldehyde fixation and cycloheximide treatment of host cells or chloramphenicol treatment of the bacteria. However, sugar depletion was not affected in flagellar export (fliQ) and quorum-sensing (luxS) gene mutants. Passaged C. jejuni showed reduced invasiveness and increased serum sensitivity in vitro. C. jejuni alters its surface polysaccharides when cocultured with epithelial cells, suggesting the existence of a cross talk mechanism that modulates CPS expression during infection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.