ReviewMethods (San Diego, Calif.)2009
Polytene chromosome squash methods for studying transcription and epigenetic chromatin modification in Drosophila using antibodies.
Review in Methods (San Diego, Calif.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed.
- Chromatin state dynamics of autosomes and the B chromosome during spermatogenesis in Pseudococcus viburni.Heredity · 2026Article
- Drosophila Keap1 Proteins Assemble Nuclear Condensates in Response to Oxidative Stress.Antioxidants (Basel, Switzerland) · 2026Article
- Structural basis of thymidine-rich DNA recognition by Drosophila P75 PWWP domain.Communications biology · 2025Article
- Article
- The catalytic-dead Pcif1 regulates gene expression and fertility inRNA (New York, N.Y.) · 2023Article
- Tip60's Novel RNA-Binding Function Modulates Alternative Splicing of Pre-mRNA Targets Implicated in Alzheimer's Disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Article
- Measuring Cellular Ploidy In Situ by Light Microscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Teflon promotes chromosomal recruitment of homolog conjunction proteins during Drosophila male meiosis.PLoS genetics · 2022Article
- Heat-induced SIRT1-mediated H4K16ac deacetylation impairs resection and SMARCAD1 recruitment to double strand breaks.iScience · 2022Article
- Drosophila Keap1 xenobiotic response factor regulates developmental transcription through binding to chromatin.Developmental biology · 2022Article
- Article
- Evolutionary conserved NSL complex/BRD4 axis controls transcription activation via histone acetylation.Nature communications · 2020Article
- Molecular effects of dADD1 misexpression in chromatin organization and transcription.BMC molecular and cell biology · 2020Article
- In vivo visualization of the i-motif DNA secondary structure in the Bombyx mori testis.Epigenetics & chromatin · 2020Article
- JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila.Nature communications · 2019Article
- Article
- TAMRA-polypyrrole for A/T sequence visualization on DNA molecules.Nucleic acids research · 2018Article
- Facultative dosage compensation of developmental genes on autosomes in Drosophila and mouse embryonic stem cells.Nature communications · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The giant polytene chromosomes from Drosophila third instar larval salivary glands provide an important model system for studying the architectural changes in chromatin morphology associated with the process of transcription initiation and elongation. Especially, analysis of the heat shock response has proved useful in correlating chromatin structure remodeling with transcriptional activity. An important tool for such studies is the labeling of polytene chromosome squash preparations with antibodies to the enzymes, transcription factors, or histone modifications of interest. However, in any immunohistochemical experiment there will be advantages and disadvantages to different methods of fixation and sample preparation, the relative merits of which must be balanced. Here we provide detailed protocols for polytene chromosome squash preparation and discuss their relative pros and cons in terms of suitability for reliable antibody labeling and preservation of high resolution chromatin structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.