ArticlePloS one2009
Roles of putative type II secretion and type IV pilus systems in the virulence of uropathogenic Escherichia coli.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 64 citations in OpenAlex.
- Article
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- Distribution of Fimbrial Genes and Their Association with Virulence and Levofloxacin Resistance/Extended-Spectrum Beta-Lactamase Production in UropathogenicAntibiotics (Basel, Switzerland) · 2025Article
- Bacterial adhesion strategies and countermeasures in urinary tract infection.Nature microbiology · 2025Review
- Gut microbiome features and resistome elements associated with colonization and infection with antibiotic-resistance threats.Gut microbes reports · 2025Article
- Nucleic acid-binding KH domain proteins influence a spectrum of biological pathways including as part of membrane-localized complexes.Journal of structural biology: X · 2024Article
- Evolutionary and functional history of the Escherichia coli K1 capsule.Nature communications · 2023Article
- Mechanisms Involved in the Active Secretion of CTX-M-15 β-Lactamase by Pathogenic Escherichia coli ST131.Antimicrobial agents and chemotherapy · 2021Article
- Genome-wide analysis inMicrobial genomics · 2021Article
- Insight Into the Virulence Related Secretion Systems, Fimbriae, and Toxins in O2:K1Frontiers in veterinary science · 2021Article
- Frequency of Antimicrobial Resistance and Class 1 and 2 Integrons inIranian journal of pharmaceutical research : IJPR · 2020Article
- Bacterial Manipulation of Wnt Signaling: A Host-Pathogen Tug-of-Wnt.Frontiers in immunology · 2019Review
- Propolis potentiates the effect of cranberry (Vaccinium macrocarpon) against the virulence of uropathogenic Escherichia coli.Scientific reports · 2018Article
- Complete genome sequence of uropathogenic Escherichia coli isolate UPEC 26-1.Genes & genomics · 2018Article
- Virulence properties of uropathogenic Escherichia coli isolated from children with urinary tract infection in Korea.Genes & genomics · 2018Article
- Comparative Genomics ofFrontiers in microbiology · 2018Article
- Expanding Role of Type II Secretion in Bacterial Pathogenesis and Beyond.Infection and immunity · 2017Review
- Structural insights into the secretin translocation channel in the type II secretion system.Nature structural & molecular biology · 2017Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundType II secretion systems (T2SS) and the evolutionarily related type IV pili (T4P) are important virulence determinants in many Gram-negative bacterial pathogens. However, the roles of T2SS and T4P in the virulence of extraintestinal pathogenic Escherichia coli have not been determined. METHODOLOGY/PRINCIPAL
findingsTo investigate the functions of putative T2SS and T4P gene clusters present in the model uropathogenic E. coli (UPEC) strains UTI89 and CFT073, we deleted the secretin gene present in each cluster. The secretin forms a channel in the outer membrane that is essential for the function of T2S and T4P systems. We compared the secretin deletion mutants with their wild type counterparts using tissue culture assays and the CBA/J mouse model of ascending urinary tract infection. No deficiencies were observed with any of the mutants in adherence, invasion or replication in human bladder or kidney cell lines, but UTI89 DeltahofQ and UTI89 DeltagspD exhibited approximately 2-fold defects in fluxing out of bladder epithelial cells. In the mouse infection model, each of the knockout mutants was able to establish successful infections in the bladder and kidneys by day one post-infection. However, UTI89 DeltahofQ and a CFT073 DeltahofQ DeltayheF double mutant both exhibited defects in colonizing the kidneys by day seven post-infection. CONCLUSIONS/SIGNIFICANCE: Based on our results, we propose that the putative T4P and T2S systems are virulence determinants of UPEC important for persistence in the urinary tract, particularly in renal tissues.
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