ArticleGenes & development2009
A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation.
Article in Genes & development, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 66 citations in OpenAlex.
- Tracing the origins of glioblastoma by investigating the role of gliogenic and related neurogenic genes/signaling pathways in GBM development: a systematic review.World journal of surgical oncology · 2022Pooled it
- A noncoding single-nucleotide polymorphism at 8q24 drivesScience (New York, N.Y.) · 2022Article
- Alternative RNA splicing in stem cells and cancer stem cells: Importance of transcript-based expression analysis.World journal of stem cells · 2021Review
- Primate-specific stress-induced transcription factor POU2F1Z protects human neuronal cells from stress.Scientific reports · 2021Article
- A comprehensive library of human transcription factors for cell fate engineering.Nature biotechnology · 2021Article
- RAR-related orphan receptor A: One gene with multiple functions related to migraine.CNS neuroscience & therapeutics · 2020Article
- Decoding the differentiation of mesenchymal stem cells into mesangial cells at the transcriptomic level.BMC genomics · 2020Article
- Development of a high-throughput arrayed neural circuitry platform using human induced neurons for drug screening applications.Lab on a chip · 2020Article
- An Improved Method for Differentiating Mouse Embryonic Stem Cells into Cerebellar Purkinje Neurons.Cerebellum (London, England) · 2019Article
- OCT4B mediates hypoxia-induced cancer dissemination.Oncogene · 2019Article
- Pou2f2 Regulates the Distribution of Dorsal Interneurons in the Mouse Developing Spinal Cord.Frontiers in molecular neuroscience · 2019Article
- Onecut Factors and Pou2f2 Regulate the Distribution of V2 Interneurons in the Mouse Developing Spinal Cord.Frontiers in cellular neuroscience · 2019Article
- Stem Cells as Potential Targets of Polyphenols in Multiple Sclerosis and Alzheimer's Disease.BioMed research international · 2018Review
- Retinoic acid-related orphan receptor alpha (RORA) variants are associated with autism spectrum disorder.Metabolic brain disease · 2017Article
- Article
- Oct-2 forms a complex with Oct-1 on the iNOS promoter and represses transcription by interfering with recruitment of RNA PolII by Oct-1.Nucleic acids research · 2015Article
- The early expansion and evolutionary dynamics of POU class genes.Molecular biology and evolution · 2014Article
- Noncoding origins of anthropoid traits and a new null model of transposon functionalization.Genome research · 2014Article
- A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.Neuron · 2014Article
- Tcf3 represses Wnt-β-catenin signaling and maintains neural stem cell population during neocortical development.PloS one · 2014Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neuronal differentiation is a complex process that involves a plethora of regulatory steps. To identify transcription factors that influence neuronal differentiation we developed a high throughput screen using embryonic stem (ES) cells. Seven-hundred human transcription factor clones were stably introduced into mouse ES (mES) cells and screened for their ability to induce neuronal differentiation of mES cells. Twenty-four factors that are capable of inducing neuronal differentiation were identified, including four known effectors of neuronal differentiation, 11 factors with limited evidence of involvement in regulating neuronal differentiation, and nine novel factors. One transcription factor, Oct-2, was studied in detail and found to be a bifunctional regulator: It can either repress or induce neuronal differentiation, depending on the particular isoform. Ectopic expression experiments demonstrate that isoform Oct-2.4 represses neuronal differentiation, whereas Oct-2.2 activates neuron formation. Consistent with a role in neuronal differentiation, Oct-2.2 expression is induced during differentiation, and cells depleted of Oct-2 and its homolog Oct-1 have a reduced capacity to differentiate into neurons. Our results reveal a number of transcription factors potentially important for mammalian neuronal differentiation, and indicate that Oct-2 may serve as a binary switch to repress differentiation in precursor cells and induce neuronal differentiation later during neuronal development.
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Registered trials
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