ArticleApplied and environmental microbiology2009
Establishment of oxidative D-xylose metabolism in Pseudomonas putida S12.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 70 citations in OpenAlex.
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- Engineering mixed sugar metabolic channels inSynthetic and systems biotechnology · 2026Article
- Engineering Saccharomyces cerevisiae for growth on xylose using an oxidative pathway.Applied microbiology and biotechnology · 2025Article
- Prospects and challenges regarding biosurfactants in advancing the petroleum industry.Frontiers in bioengineering and biotechnology · 2025Review
- Metabolic bottlenecks ofMetabolic engineering communications · 2024Article
- Revealing oxidative pentose metabolism in new Pseudomonas putida isolates.Environmental microbiology · 2023Article
- Herbaspirillum seropedicae expresses non-phosphorylative pathways for D-xylose catabolism.Applied microbiology and biotechnology · 2021Article
- Synthesis of aromatic amino acids from 2G lignocellulosic substrates.Microbial biotechnology · 2021Article
- The anoxic electrode-driven fructose catabolism of Pseudomonas putida KT2440.Microbial biotechnology · 2021Article
- Industrial biotechnology of Pseudomonas putida: advances and prospects.Applied microbiology and biotechnology · 2020Review
- Heterologous expression of genes for bioconversion of xylose to xylonic acid in Corynebacterium glutamicum and optimization of the bioprocess.AMB Express · 2020Article
- A combined experimental and modelling approach for the Weimberg pathway optimisation.Nature communications · 2020Article
- Alone at last! - Heterologous expression of a single gene is sufficient for establishing the five-step Weimberg pathway inMetabolic engineering communications · 2019Article
- Improved cell growth and biosynthesis of glycolic acid by overexpression of membrane-bound pyridine nucleotide transhydrogenase.Journal of industrial microbiology & biotechnology · 2019Article
- Comparison of Isomerase and Weimberg Pathway for γ-PGA Production From Xylose by EngineeredFrontiers in bioengineering and biotechnology · 2019Article
- Comparison of Three Xylose Pathways inFrontiers in bioengineering and biotechnology · 2019Article
- Exploring D-xylose oxidation in Saccharomyces cerevisiae through the Weimberg pathway.AMB Express · 2018Article
- Development of a high efficiency integration system and promoter library for rapid modification ofMetabolic engineering communications · 2017Article
- Crystallization and X-ray diffraction analysis of an L-arabinonate dehydratase from Rhizobium leguminosarum bv. trifolii and a D-xylonate dehydratase from Caulobacter crescentus.Acta crystallographica. Section F, Structural biology communications · 2016Article
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The oxidative D-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on D-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g D-xylose(-1)) and a maximum growth rate of 0.21 h(-1). Remarkably, most of the genes of the xylXABCD operon appeared to be dispensable for growth on D-xylose. Only the xylD gene, encoding D-xylonate dehydratase, proved to be essential for establishing an oxidative D-xylose catabolic pathway in P. putida S12. The growth performance on D-xylose was, however, greatly improved by coexpression of xylXA, encoding 2-keto-3-deoxy-D-xylonate dehydratase and alpha-ketoglutaric semialdehyde dehydrogenase, respectively. The endogenous periplasmic glucose dehydrogenase (Gcd) of P. putida S12 was found to play a key role in efficient oxidative D-xylose utilization. Gcd activity not only contributes to D-xylose oxidation but also prevents the intracellular accumulation of toxic catabolic intermediates which delays or even eliminates growth on D-xylose.
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