ArticleJournal of bacteriology2009
The cin and rai quorum-sensing regulatory systems in Rhizobium leguminosarum are coordinated by ExpR and CinS, a small regulatory protein coexpressed with CinI.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
- Review
- Article
- Comparative genome analysis ofMicrobial genomics · 2023Article
- Rhizobial Exopolysaccharides: Genetic Regulation of Their Synthesis and Relevance in Symbiosis with Legumes.International journal of molecular sciences · 2021Review
- Naphthoquinones inhibit formation and viability of Yersinia enterocolitica biofilm.World journal of microbiology & biotechnology · 2021Article
- Regulation Mediated by N-Acyl Homoserine Lactone Quorum Sensing Signals in the Rhizobium-Legume Symbiosis.Genes · 2018Review
- Conserved small mRNA with an unique, extended Shine-Dalgarno sequence.RNA biology · 2017Article
- Transcriptional analysis of genes involved in competitive nodulation in Bradyrhizobium diazoefficiens at the presence of soybean root exudates.Scientific reports · 2017Article
- Transcriptome analysis revealed that a quorum sensing system regulates the transfer of the pAt megaplasmid in Agrobacterium tumefaciens.BMC genomics · 2016Article
- Plant growth-promoting bacteria as inoculants in agricultural soils.Genetics and molecular biology · 2015Article
- The quorum sensing regulator CinR hierarchically regulates two other quorum sensing pathways in ligand-dependent and -independent fashions in Rhizobium etli.Journal of bacteriology · 2015Article
- Mutation of praR in Rhizobium leguminosarum enhances root biofilms, improving nodulation competitiveness by increased expression of attachment proteins.Molecular microbiology · 2014Article
- LuxR- and luxI-type quorum-sensing circuits are prevalent in members of the Populus deltoides microbiome.Applied and environmental microbiology · 2013Article
- Comparative genomic analysis of six bacteria belonging to the genus Novosphingobium: insights into marine adaptation, cell-cell signaling and bioremediation.BMC genomics · 2013Article
- Gut and root microbiota commonalities.Applied and environmental microbiology · 2013Review
- Lon protease of Azorhizobium caulinodans ORS571 is required for suppression of reb gene expression.Applied and environmental microbiology · 2012Article
- A unique regulator contributes to quorum sensing and virulence in Burkholderia cenocepacia.PloS one · 2012Article
- Adaptation of Rhizobium leguminosarum to pea, alfalfa and sugar beet rhizospheres investigated by comparative transcriptomics.Genome biology · 2011Article
- Environmental signals and regulatory pathways that influence exopolysaccharide production in rhizobia.International journal of molecular sciences · 2011Review
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
To understand how the Rhizobium leguminosarum raiI-raiR quorum-sensing system is regulated, we identified mutants with decreased levels of RaiI-made N-acyl homoserine lactones (AHLs). A LuxR-type regulator, ExpR, is required for raiR expression, and RaiR is required to induce raiI. Since raiR (and raiI) expression is also reduced in cinI and cinR quorum-sensing mutants, we thought CinI-made AHLs may activate ExpR to induce raiR. However, added CinI-made AHLs did not induce raiR expression in a cinI mutant. The reduced raiR expression in cinI and cinR mutants was due to lack of expression of cinS immediately downstream of cinI. cinS encodes a 67-residue protein, translationally coupled to CinI, and cinS acts downstream of expR for raiR induction. Cloned cinS in R. leguminosarum caused an unusual collapse of colony structure, and this was delayed by mutation of expR. The phenotype looked like a loss of exopolysaccharide (EPS) integrity; mutations in cinI, cinR, cinS, and expR all reduced expression of plyB, encoding an EPS glycanase, and mutation of plyB abolished the effect of cloned cinS on colony morphology. We conclude that CinS and ExpR act to increase PlyB levels, thereby influencing the bacterial surface. CinS is conserved in other rhizobia, including Rhizobium etli; the previously observed effect of cinI and cinR mutations decreasing swarming in that strain is primarily due to a lack of CinS rather than a lack of CinI-made AHL. We conclude that CinS mediates quorum-sensing regulation because it is coregulated with an AHL synthase and demonstrate that its regulatory effects can occur in the absence of AHLs.
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