Evidence map›Paper›PMID 19270085›Full record

ArticleJournal of bacteriology2009

Structural characterization of an oligosaccharide made by Neisseria sicca.

Ellen T O'Connor, Hui Zhou, Kevin Bullock, Karen V Swanson, J McLeod Griffiss, Vernon N Reinhold, Clinton J Miller, Daniel C Stein

Open access · greenAbstract read
In one paragraph

Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Ellen T O'ConnorDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA.
Hui Zhou
Kevin Bullock
Karen V Swanson
J McLeod Griffiss
Vernon N Reinhold
Clinton J Miller
Daniel C Stein
University of Maryland, College Park · USUniversity of New Hampshire · USUniversity of California, San Francisco · US

Funding

Risk Factors and Susceptibility in Gonococcal InfectionR01AI065605 · NIAID · NORTHERN CALIFORNIA INSTITUTE/RES/EDU · PI GRIFFISS, JOHN MCLEOD · 2007 to 2011
$2.1M
LIPOOLIGOSACCHARIDE BIOSYNTHESIS IN NEISSERIACEAER01AI024452 · NIAID · UNIVERSITY OF MARYLAND COLLEGE PK CAMPUS · PI STEIN, DANIEL C · 1995 to 2004
$1.3M
IMMUNOCHEMISTRY OF GONOCOCCAL LIPOPOLYSACCHARIDER01AI021620 · NIAID · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI GRIFFISS, JOHN MCLEOD · 1985 to 2003
$1.1M
Lipooligosaccharide biosynthesis in NeisseriaceaeR56AI024452 · NIAID · UNIV OF MARYLAND, COLLEGE PARK · PI STEIN, DANIEL C · 2006 to 2006
$359k
LIPOOLIGOSACCHARIDE BIOSYNTHESIS IN NEISSERIACEAER29AI024452 · NIAID · UNIVERSITY OF MARYLAND COLLEGE PK CAMPUS · PI STEIN, DANIEL C · 1988 to 1992
–
NIAID NIH HHS AI 065605NIAID NIH HHS AI 21620NIAID NIH HHS AI 24452NIAID NIH HHS R01 AI021620NIAID NIH HHS R01 AI024452NIAID NIH HHS R01 AI065605NIAID NIH HHS R56 AI024452
6 · The paper itself

Abstract

Neisseria sicca 4320 expresses two carbohydrate-containing components with sodium dodecyl sulfate-polyacrylamide gel electrophoresis mobilities that resemble those of lipooligosaccharide and lipopolysaccharide. Using matrix-assisted laser desorption ionization--time of flight and electrospray ionization mass spectrometry, we characterized a disaccharide carbohydrate repeating unit expressed by this strain. Gas chromatography identified the sugars composing the unit as rhamnose and N-acetyl-D-glucosamine. Glycosidase digestion confirmed the identity of the nonreducing terminal sugar of the disaccharide and established its beta-anomeric configuration. Mass spectrometry analysis and lectin binding were used to verify the linkages within the disaccharide repeat. The results revealed that the disaccharide repeat is [-4) beta-L-rhamnose (1-3) beta-N-acetyl-D-glucosamine (1-] with an N-acetyl-D-glucosamine nonreducing terminus. This work is the first structural characterization of a molecule that possesses rhamnose in the genus Neisseria.

Indexed as

AcetylglucosamineBlotting, WesternChromatography, GasDisaccharidesElectrophoresis, Polyacrylamide GelGas Chromatography-Mass SpectrometryMolecular StructureNeisseria siccaOligosaccharidesRhamnoseSpectrometry, Mass, Electrospray IonizationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationAcetylglucosamineDisaccharidesOligosaccharidesRhamnose

Identifiers

PMID19270085
PMCPMC2687153
OpenAlexW2147371480

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.