ArticleThe Journal of biological chemistry2009
Initiation of hepatitis C virus infection requires the dynamic microtubule network: role of the viral nucleocapsid protein.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.
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Who cites it
44 citing papers in PubMed, 1 synthesis or guideline pooled it, 73 citations in OpenAlex.
- An integrated transcriptomic and meta-analysis of hepatoma cells reveals factors that influence susceptibility to HCV infection.PloS one · 2011Pooled it
- Hijacking the cytoskeleton: association of HCV polymerase with α/β-tubulin and its potential relation to the viral replication and cell proliferation.Molecular and cellular biochemistry · 2026Article
- Synergistic inhibition of hepatitis C virus infection by a novel microtubule inhibitor in combination with daclatasvir.Biochemistry and biophysics reports · 2022Article
- Unraveling the Dynamic Role of Microtubules in the HBV Life Cycle.Journal of clinical and translational hepatology · 2022Article
- Review
- A rice protein interaction network reveals high centrality nodes and candidate pathogen effector targets.Computational and structural biotechnology journal · 2022Article
- PIAS1 Regulates Hepatitis C Virus-Induced Lipid Droplet Accumulation by Controlling Septin 9 and Microtubule Filament Assembly.Pathogens (Basel, Switzerland) · 2021Article
- Comparative Analysis of Hepatitis C Virus NS5A Dynamics and Localization in Assembly-Deficient Mutants.Pathogens (Basel, Switzerland) · 2021Article
- Integrative Network Biology Framework Elucidates Molecular Mechanisms of SARS-CoV-2 Pathogenesis.iScience · 2020Article
- Integrative Network Biology Framework Elucidates Molecular Mechanisms of SARS-CoV-2 Pathogenesis.SSRN · 2020Article
- Article
- Construction and analysis of a comprehensive protein interaction network of HCV with its host Homo sapiens.BMC infectious diseases · 2019Article
- Exploitation of Cytoskeletal Networks during Early Viral Infection.Trends in microbiology · 2019Review
- Sirtuin 2 Isoform 1 Enhances Hepatitis B Virus RNA Transcription and DNA Synthesis through the AKT/GSK-3β/β-Catenin Signaling Pathway.Journal of virology · 2018Article
- Oncogenic Signaling Induced by HCV Infection.Viruses · 2018Review
- Viral manipulation of STAT3: Evade, exploit, and injure.PLoS pathogens · 2018Review
- Rewiring cellular networks by members of the Flaviviridae family.Nature reviews. Microbiology · 2018Review
- Microtubule Regulation and Function during Virus Infection.Journal of virology · 2017Review
- Distinct functions of diaphanous-related formins regulate HIV-1 uncoating and transport.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Wsv023 interacted withRoyal Society open science · 2017Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
Abstract
Early events leading to the establishment of hepatitis C virus (HCV) infection are not completely understood. We show that intact and dynamic microtubules play a key role in the initiation of productive HCV infection. Microtubules were required for virus entry into cells, as evidenced using virus pseudotypes presenting HCV envelope proteins on their surface. Studies carried out using the recent infectious HCV model revealed that microtubules also play an essential role in early, postfusion steps of the virus cycle. Moreover, low concentrations of vinblastin and nocodazol, microtubule-affecting drugs, and paclitaxel, which stabilizes microtubules, inhibited infection, suggesting that microtubule dynamic instability and/or treadmilling mechanisms are involved in HCV internalization and early transport. By protein chip and direct core-dependent pull-down assays, followed by mass spectrometry, we identified beta- and alpha-tubulin as cellular partners of the HCV core protein. Surface plasmon resonance analyses confirmed that core directly binds to tubulin with high affinity via amino acids 2-117. The interaction of core with tubulin in vitro promoted its polymerization and enhanced the formation of microtubules. Immune electron microscopy showed that HCV core associates, at least temporarily, with microtubules polymerized in its presence. Studies by confocal microscopy showed a juxtaposition of core with microtubules in HCV-infected cells. In summary, we report that intact and dynamic microtubules are required for virus entry into cells and for early postfusion steps of infection. HCV may exploit a direct interaction of core with tubulin, enhancing microtubule polymerization, to establish efficient infection and promote virus transport and/or assembly in infected cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.