ArticleThe Journal of biological chemistry2009
Calpain expression and activity during lens fiber cell differentiation.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 46 citations in OpenAlex.
- Periaxin gene variants are linked to age-related cataracts in Cx46 deficient lenses.Communications biology · 2025Article
- The effect of fibre cell remodelling on the power and optical quality of the lens.Journal of the Royal Society, Interface · 2023Article
- Article
- Methodologies to unlock the molecular expression and cellular structure of ocular lens epithelial cells.Frontiers in cell and developmental biology · 2022Article
- [Calpain activation promotes dialysis-associated peritoneal fibrosis in rats].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2021Article
- Mutation of the TRPM3 cation channel underlies progressive cataract development and lens calcification associated with pro-fibrotic and immune cell responses.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Spatial Analysis of Single Fiber Cells of the Developing Ocular Lens Reveals Regulated Heterogeneity of Gene Expression.iScience · 2018Article
- Calpain and spectrin breakdown products as potential biomarkers in tuberculous pleural effusion.Journal of thoracic disease · 2018Article
- The lens actin filament cytoskeleton: Diverse structures for complex functions.Experimental eye research · 2017Review
- The cause and consequence of fiber cell compaction in the vertebrate lens.Experimental eye research · 2017Review
- Disassembly of the lens fiber cell nucleus to create a clear lens: The p27 descent.Experimental eye research · 2017Review
- Lens transcriptome profile during cataract development in Mip-null mice.Biochemical and biophysical research communications · 2016Article
- Lens ER-stress response during cataract development in Mip-mutant mice.Biochimica et biophysica acta · 2016Article
- Activation of calpain by renin-angiotensin system in pleural mesothelial cells mediates tuberculous pleural fibrosis.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Birc7: A Late Fiber Gene of the Crystalline Lens.Investigative ophthalmology & visual science · 2015Article
- Lens ion homeostasis relies on the assembly and/or stability of large connexin 46 gap junction plaques on the broad sides of differentiating fiber cells.American journal of physiology. Cell physiology · 2015Article
- Inhibition of calpain prevents manganese-induced cell injury and alpha-synuclein oligomerization in organotypic brain slice cultures.PloS one · 2015Article
- Ultrastructural analysis of the human lens fiber cell remodeling zone and the initiation of cellular compaction.Experimental eye research · 2013Article
- p62 expression and autophagy in αB-crystallin R120G mutant knock-in mouse model of hereditary cataract.Experimental eye research · 2013Article
- Monoclonal antibody 10A5 recognizes an antigen unique to the water-insoluble 25/45 membrane fraction of the rat ocular lens.SpringerPlus · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In animal models, the dysregulated activity of calcium-activated proteases, calpains, contributes directly to cataract formation. However, the physiological role of calpains in the healthy lens is not well defined. In this study, we examined the expression pattern of calpains in the mouse lens. Real time PCR and Western blotting data indicated that calpain 1, 2, 3, and 7 were expressed in lens fiber cells. Using controlled lysis, depth-dependent expression profiles for each calpain were obtained. These indicated that, unlike calpain 1, 2, and 7, which were most abundant in cells near the lens surface, calpain 3 expression was strongest in the deep cortical region of the lens. We detected calpain activities in vitro and showed that calpains were active in vivo by microinjecting fluorogenic calpain substrates into cortical fiber cells. To identify endogenous calpain substrates, membrane/cytoskeleton preparations were treated with recombinant calpain, and cleaved products were identified by two-dimensional difference electrophoresis/mass spectrometry. Among the calpain substrates identified by this approach was alphaII-spectrin. An antibody that specifically recognized calpain-cleaved spectrin was used to demonstrate that spectrin is cleaved in vivo, late in fiber cell differentiation, at or about the time that lens organelles are degraded. The generation of the calpain-specific spectrin cleavage product was not observed in lens tissue from calpain 3-null mice, indicating that calpain 3 is uniquely activated during lens fiber differentiation. Our data suggest a role for calpains in the remodeling of the membrane cytoskeleton that occurs with fiber cell maturation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.