ArticleBMC molecular biology2009
Cloning-free regulated monitoring of reporter and gene expression.
Article in BMC molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Post-transcriptional regulation of BIRC5/survivin expression and induction of apoptosis in breast cancer cells by tristetraprolin.RNA biology · 2024Article
- Beyond the exome: utility of long-read whole genome sequencing in exome-negative autosomal recessive diseases.Genome medicine · 2023Article
- Identification of a novel genetic locus underlying tremor and dystonia.Human genomics · 2017Article
- A novel mechanism for variable phenotypic expressivity in Mendelian diseases uncovered by an AU-rich element (ARE)-creating mutation.Genome biology · 2017Article
- Human Cytokinome Analysis for Interferon Response.Journal of virology · 2015Article
- miR-29a inhibition normalizes HuR over-expression and aberrant AU-rich mRNA stability in invasive cancer.The Journal of pathology · 2013Article
- Proteomics analysis of human obesity reveals the epigenetic factor HDAC4 as a potential target for obesity.PloS one · 2013Article
- Regulation of p21/CIP1/WAF-1 mediated cell-cycle arrest by RNase L and tristetraprolin, and involvement of AU-rich elements.Nucleic acids research · 2012Article
- UU/UA dinucleotide frequency reduction in coding regions results in increased mRNA stability and protein expression.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
- Green fluorescent protein reporter system with transcriptional sequence heterogeneity for monitoring the interferon response.Journal of virology · 2011Article
- A versatile ribosomal protein promoter-based reporter system for selective assessment of RNA stability and post-transcriptional control.RNA (New York, N.Y.) · 2010Article
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe majority of the promoters, their regulatory elements, and their variations in the human genome remain unknown. Reporter gene technology for transcriptional activity is a widely used tool for the study of promoter structure, gene regulation, and signaling pathways. Construction of transcriptional reporter vectors, including use of cis-acting sequences, requires cloning and time-demanding manipulations, particularly with introduced mutations.
resultsIn this report, we describe a cloning-free strategy to generate transcriptionally-controllable linear reporter constructs. This approach was applied in common transcriptional models of inflammatory response and the interferon system. In addition, it was used to delineate minimal transcriptional activity of selected ribosomal protein promoters. The approach was tested for conversion of genes into TetO-inducible/repressible expression cassettes.
conclusionThe simple introduction and tuning of any transcriptional control in the linear DNA product renders promoter activation and regulated gene studies simple and versatile.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.