ArticleBMC genetics2009
A mouse chromosome 4 balancer ENU-mutagenesis screen isolates eleven lethal lines.
Article in BMC genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- An efficient i-GONAD method for creating and maintaining lethal mutant mice using an inversion balancer identified from the C3H/HeJJcl strain.G3 (Bethesda, Md.) · 2021Article
- The Role of Cullin-RING Ligases in Striated Muscle Development, Function, and Disease.International journal of molecular sciences · 2020Review
- High-throughput mouse phenomics for characterizing mammalian gene function.Nature reviews. Genetics · 2018Review
- Generation of a Mouse Full-length Balancer with Versatile Cassette-shuttling Selection Strategy.International journal of biological sciences · 2016Article
- Mouse Genome Database: From sequence to phenotypes and disease models.Genesis (New York, N.Y. : 2000) · 2015Review
- Going forward with genetics: recent technological advances and forward genetics in mice.The American journal of pathology · 2013Review
- Article
- Mouse large-scale phenotyping initiatives: overview of the European Mouse Disease Clinic (EUMODIC) and of the Wellcome Trust Sanger Institute Mouse Genetics Project.Mammalian genome : official journal of the International Mammalian Genome Society · 2012Review
- Article
- Sex-dependent gene regulatory networks of the heart rhythm.Functional & integrative genomics · 2010Article
- Mouse mutagenesis with the chemical supermutagen ENU.Methods in enzymology · 2010Article
Corrections and comments
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Authors and funding
12 authors at 7 institutions in 2 countries.
Funding
Abstract
backgroundENU-mutagenesis is a powerful technique to identify genes regulating mammalian development. To functionally annotate the distal region of mouse chromosome 4, we performed an ENU-mutagenesis screen using a balancer chromosome targeted to this region of the genome.
resultsWe isolated 11 lethal lines that map to the region of chromosome 4 between D4Mit117 and D4Mit281. These lines form 10 complementation groups. The majority of lines die during embryonic development between E5.5 and E12.5 and display defects in gastrulation, cardiac development, and craniofacial development. One line displayed postnatal lethality and neurological defects, including ataxia and seizures.
conclusionThese eleven mutants allow us to query gene function within the distal region of mouse chromosome 4 and demonstrate that new mouse models of mammalian developmental defects can easily and quickly be generated and mapped with the use of ENU-mutagenesis in combination with balancer chromosomes. The low number of mutations isolated in this screen compared with other balancer chromosome screens indicates that the functions of genes in different regions of the genome vary widely.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.