ArticleCerebrospinal fluid research2009
Molecular diagnosis of Toxoplasma gondii infection in cerebrospinal fluid from AIDS patients.
Article in Cerebrospinal fluid research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 53 citations in OpenAlex.
- Hemichorea-Hemiballismus As Presentation of Toxoplasma gondii Encephalitis in an Immunocompromised Host.Cureus · 2026Article
- Elevated Liver Enzymes and Genotype I of T. gondii Among Liver Cancer Patients: Evidence of a Potential Link.Asian Pacific journal of cancer prevention : APJCP · 2026Article
- Diagnostic Challenges and Treatment of Concurrent Toxoplasmosis and Disseminated Cryptococcus in an Immunocompromised Patient.Case reports in infectious diseases · 2025Article
- Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections.Clinical microbiology reviews · 2024Review
- Central nervous system aspergillosis misdiagnosed as Toxoplasma gondii encephalitis in a patient with AIDS: a case report.AIDS research and therapy · 2022Article
- Serological Responses to Toxoplasma gondii and Matrix Antigen 1 Predict the Risk of Subsequent Toxoplasmic Encephalitis in People Living With Human Immunodeficiency Virus (HIV).Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Seroprevalence of Toxoplasma gondii and associated risk factors in domestic pigs raised from Cuba.Parasitology research · 2021Article
- Murine Model of Primary Acquired Ocular Toxoplasmosis: Fluorescein Angiography and Multiplex Immune Mediator Profiles in the Aqueous Humor.Investigative ophthalmology & visual science · 2021Article
- Development of a Novel Protocol Based on Blood Clot to Improve the Sensitivity of qPCR Detection ofThe American journal of tropical medicine and hygiene · 2019Article
- Prevalence of Toxoplasma gondii infection in HIV-infected patients and food animals and direct genotyping of T. gondii isolates, Southern Ghana.Parasitology research · 2017Article
- Molecular diagnosis of Toxoplasma gondii infection in Libya.BMC infectious diseases · 2016Article
- Detection of toxoplasmosis in patients with end-stage renal disease by enzyme-linked immunosorbent assay and polymerase chain reaction methods.Parasitology research · 2013Article
- Epidemiology of and diagnostic strategies for toxoplasmosis.Clinical microbiology reviews · 2012Review
- Evaluation of real-time polymerase chain reaction for the diagnosis of protozoal myeloencephalitis in horses using cerebrospinal fluid.Journal of veterinary internal medicineArticle
- HIV-Related Cerebral Toxoplasmosis Revisited: Current Concepts and Controversies of an Old Disease.Journal of the International Association of Providers of AIDS CareReview
- Solitary hypothalamopituitary toxoplasmosis abscess in a patient with AIDS.AJNR. American journal of neuroradiologyArticle
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundToxoplasmic encephalitis (TE) is one of the most common opportunistic infections in immunocompromised patients. In Cuba, despite the highly active antiretroviral therapy, TE is still the most important cause of cerebral mass lesions in patients infected with the human immunodeficiency virus (HIV). The detection of Toxoplasma gondii by PCR may facilitate the diagnosis and follow-up of TE in acquired immunodeficiency syndrome (AIDS) patients by direct identification of parasite DNA in clinical samples. The aim of the present study was to evaluate a rapid PCR method using the B1 gene to detect T. gondii in cerebrospinal fluid (CSF) samples from patients with suspected TE.
methodsCSF samples from AIDS and HIV-negative patients were analyzed. Patients were divided into two groups according to the Centre for Disease Control and Prevention (CDC) criteria for AIDS-related TE: AIDS patients with suspected neurotoxoplasmosis and AIDS and HIV-negative patients with other confirmed neurological diseases but no suspicions of TE. Predictive values, diagnostic accuracy, sensitivity and specificity of the PCR B1 method were calculated.
resultsThe results obtained from 190 patients showed that this assay has a good sensitivity and specificity (83.3% and 95.7%, respectively) for the diagnosis of TE in AIDS patients.
conclusionPCR using the B1 gene and B22/B23 set of primers is a single, rapid and reliable method that may be valuable for discrimination between toxoplasmosis and other central nervous system (CNS) diseases.
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Registered trials
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